FastQCFastQC Report
Wed 28 Aug 2024
HWTCTDMXY_n01_CITE-seq_test_TCells_387D0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTCTDMXY_n01_CITE-seq_test_TCells_387D0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17534255
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACCTTCCGATCTTTCTCAACAATA1604210.9149005760438639No Hit
ACACGACGCTCTTCCGATCTACACGA1223580.6978226334680316No Hit
GAATGAATCACATGCATTGACCGGGG981530.5597785591688954No Hit
AGTTGGTTGATTGTAGATATTGGGCT688220.392500280165881No Hit
GGACTTTAATCGTTGAACAAACGAAC667880.3809001294893909No Hit
GGTGAAGTCTTAGCATGTACTGCTCG661410.3772102093872822No Hit
ATCTGCCTGATACTCGCCAGCTGAGC612900.3495443633048567No Hit
CTACATTAGTTACACAACCCCAAAGA606380.34582592759144887No Hit
GCTCCTAACCAACAAACTTAAAATAA580190.3308894503929594No Hit
TTCTCAACAATAGCGGGGTGAGACAA566140.32287656361790107No Hit
ACTCCTAAGAAGGTTTTACCAATACG547740.3123828186598176No Hit
TTAACTCGTTGGAGGTCGCTTAGTGA545380.3110368818064982No Hit
GGATGTTAGGATTCCGTTGGCCCGTA538350.30702758685783915No Hit
AACTTTCAGTGCTGCCAGGGGGCAAT532370.303617119746462No Hit
GTGCTTCTCCACGACGAAGATGAGGA526750.3004119650364387No Hit
GGATTACTCCGGTCTGAACTCAGATC523930.29880368455916717No Hit
TGCTCTCGCGAGGTCGCTTCTCTTTG516270.29443509290813896No Hit
TTAGGCACAGCTGTATCGCTAACTGG487540.27805002265565315No Hit
CTTCTTCTGCGCGCAGCGGATAGGGA450940.2571765951846828No Hit
TGGTTCCCATCGGAAGCGCATATTCC433620.24729878743066075No Hit
GGCTGGTTGTTAAGCGTACACCGGCC433080.24699081882863No Hit
GGACTTGTTGGTTGATTGTAGATATT421250.24024402519525354No Hit
GCTTCCAGAAGAACCACAAACAGAGC409480.23353145029543598No Hit
ATTGGACCAGAAGCACATACCTAGCT400250.2282674684496148No Hit
GCATACATCACTATTCTTAACTCTTG399870.22805074980374132No Hit
GGGACCTCCACGACGTCTTACCGACG391990.22355668946299687No Hit
ACTATCTCATCCGTGGAGCTTCCCTG387410.22094465946799566No Hit
AATTGTCAGTTCAGTGTTTTAATCTG380020.21673005211798277No Hit
TGCGTGGTCCATTCTAGTGGGGCGTC378970.21613122428070083No Hit
GCTTCCACCCTCTGTATTACCGAGGC373260.21287474147033905No Hit
TTCTCAACACCCATGGCTGGGTTCGT363840.2075024003015811No Hit
AGGGATGTCCTGATCCAACATCGAGG363540.20733130663378624No Hit
GGCATTAGTTAGCGGAACCCGTGTTT363180.20712599423243247No Hit
AACGTTGCATCCCATCTCCACACAGG361400.20611083847018305No Hit
TGCGTGGTCCATTCTAGTGGAGCATC361190.20599107290272667No Hit
TGGGAAGTCCAGTAGTGATCGGAAAG357250.20374404273235447No Hit
GTAGGCCCTCCTGGCTTGTGGTTCCT354940.2024266214903342No Hit
AACTCCCGGAGTTCACTCAAACTCAT353960.20186771550887106No Hit
CGGACACGTGCAACTTCTCGACTCGA348600.19881084197760326No Hit
GTAGGCCTTATTTCTCTTGTCCTTTC347960.19844584215297426No Hit
CACCATTGGCTCCATTACCCCCACAA347200.19801240486122737No Hit
CATCAGAGGTGGGCTTCACACTTAGA347030.19791545178281028No Hit
GGAACTTAGGTTCCTAAGGCGGCTGG334550.19079795520254497No Hit
GGGAATGTTCCTCTTTCAGTATCCTA331440.18902428417973846No Hit
AAAGTAGCAGGTTTCAGCGCATAGTC331340.18896725295714017No Hit
TTGCATGAAATGTTCCCTTGGTATCT330250.18834561263081892No Hit
ATAACGCCATGAACCTAGACTCTCAG329920.1881574095962446No Hit
ACAGTGTTTAGAAAAGCCAGGCAGAA326190.18603014499332876No Hit
CATCCACTCATTTGGGGCCCTGCTGC324640.18514616104305545No Hit
TCACAGATGCACAATGTGAGGCTTAG320000.18249991231449525No Hit
TACTTACCAAGCGCTCTGCTGAATGT319660.1823060061576611No Hit
TTGCCGTGTTGGGTTGACAGTGAGGG315810.18011030408762735No Hit
ATTTACTTAAGTTTCAGCCTTGCGAC315390.17987077295271456No Hit
ATTCTACAGCTACCTAACATGGCCTC314950.17961983557328212No Hit
ATCACGAGTACGACCCCTGGCTCGCA312730.17835374243160032No Hit
TGAAAGATCATAGCACGTAGGCCGGA312330.1781256175412072No Hit
GTAGGCCTACTATGGGTGTTAAATTT310520.17709335241217833No Hit
GGCTGGTTTCGTATTAAACAACATGC308540.1759641342047324No Hit
GAACTTGCGCTTACTTTGTGGCCTTC308250.1757987436591974No Hit
TTTACTGATATAAAATCATGGGAAAT307580.1754166344677889No Hit
GCATGCGCAGGGCATACCCCCCTTCA306670.17489765034214455No Hit
CTAATGGTCGCCAGCAACACAGGGCT305960.1744927286616968No Hit
AATCGGTTCTGTCTATGCATTCCTGA304580.17370569778984052No Hit
GACTGGAGAATCCCAGGGACGGGGGA304520.17367147905628155No Hit
TTCTCAACAATAGCGGCCGCACAGGA299490.17080280855958807No Hit
ACATCAGCATGGAATACATCCAAACG295010.16824780978718515No Hit
ATGTGTGTCTATGTGTGTGTGTGTGT288140.16432976479468334No Hit
ATAGCGTATATGTGTCAATCCCAATC286390.16333171839921343No Hit
CAAGATCAGCACCGCTGATAGGACAG285900.16305226540848186No Hit
GGAACTTAGGTTCCTAGGGTGTCTGT285770.1629781248191041No Hit
GCCTCTAGGCTCGACGCCGGAATATA285480.16281273427356907No Hit
ACTGCTCCACGAAAGCAGCTTGTGCC285230.16267015621707337No Hit
TGAGCATCACCAACCGTACAGACTTC284690.16236218761504267No Hit
GGTGCGTGCGGCCCAGAACATCTAAG282560.1611474225736993No Hit
GCTCCTAAGGATATACTCGTTGCATT281480.16053148536963788No Hit
AACCATGCAGGCAGTACCTCTATCAT273750.15612297186279087No Hit
GACGTTATCACCCTCACTTAGGTTGG272720.15553555027002858No Hit
CCTTCAGAATGGACTGGTTGGATCTC272440.1553758628467534No Hit
AGAGGAGTTCAGTTATATGTTTGGGA271900.15506789424472267No Hit
TTCTCAACAATAGCGGCGGTCATTGC270860.15447476952970057No Hit
GGCTGGTGGGAGTTGACGATGAGTAT270040.15400711350439467No Hit
TTACTGGTAGCCTCGTTCGTCCTCGT268940.1533797700558136No Hit
TACTATACAGGTTGCCACAGCTTCGG267530.15257562981717787No Hit
GCCTCTATCCAAACACTGGTCTGTAC267210.15239312990486337No Hit
GGGTACCTCTGAAATCAGAGGTACCC266730.15211938003639164No Hit
AACCATGGTGCGCACCGAGGGCCCCC265880.15163461464430625No Hit
GTCACAAGTCAACTGTATTCCACAGC265380.15134945853131485No Hit
GTAACGTCAGTAAGATCCATCGGGTT265260.1512810210641969No Hit
TAGACCACAGGCCAACAGCTGCCAAC264610.1509103181173081No Hit
GGAATTACTCCGGTCTGAACTCAGAT263720.15040274023618339No Hit
GGACTGTTAATTGTCAGTTCAGTGTT263530.15029438091324665No Hit
GCATGTATCCGCATAAGGCTTATAAT262650.1497925061543818No Hit
ACCAGTATCCGCGTTTGACCCCAGGG262450.14967844370918523No Hit
GTGAAGGCAGCGAACAGCGGAAACCC259180.1478135227302215No Hit
GCAAACTTCACCCTCATCCGGCATCA259020.14772227277406424No Hit
CTGTGCTCATCGGACCGATTATTGCA258580.14747133539463184No Hit
GCAAACTTCACCCTCACGCCAGTCAC257240.14670711701181485No Hit
GGAACTTTCACTATTCCCTTCCGCCG255420.1456691487605262No Hit
ACGGGCTAGGCATGCTCATAAGGAAA252980.14427758692912818No Hit
AAGGTTCCAGGAATGCACTTCACAAG252840.14419774321749054No Hit
TTCTACAAGATGTGTAGCCCTTTTGC252760.14415211823941193No Hit
ATTACTCTGGGGTGACAAGTAATGCA252250.14386125900416072No Hit
TAACGAAAAGGTGGCGGGTTCAGATG244930.13968657350996663No Hit
GACGCAGGCTTATGCGGAGGAGAATG244340.13935008929663678No Hit
GCTCCTAGAGGATGCTCTCTCTTCCT243090.13863719901415827No Hit
GACTGCGCACAGACAGTTGTTTATCC242380.1382322773337105No Hit
GGGGATTTTTTAGGTAGTGGGTGTTG242100.13807258991043533No Hit
GACAGAGTCATGTGGTCTGTCTGCTG241970.13799844932105756No Hit
TCAACGAGTAAATACGCAGATCCACG240790.13732548089439786No Hit
CGTCGTTATCGGAATTAACCAGACAA240690.13726844967179958No Hit
GCACAGGCCTGCGAGATCCTTTTCAA239650.13667532495677748No Hit
TGTTCCGGCGAGCGGGCTTTTCACCC239450.1365612625115809No Hit
TACTCATTCTAAGCCACGCCTCAGGA238640.13609930960853484No Hit
AGGGATGTCTGGGCCACAATCAAATG237960.13571149729486653No Hit
CTGAAGTGTGCAGACAGACTCATCGG237910.13568298168356738No Hit
GACTACTGGAAAAAACATAGCCTTGA236850.13507845072402563No Hit
TACGGTAGTGTTGGGATATACTTCGG235570.13434845107476764No Hit
GCCACAACTACTGAGCCCACATTCCC234850.13393782627206002No Hit
GCCTCTATCCAAACACCCACGCCGAT234830.13392642002754038No Hit
TCACTCGCATCAACTCCACCACACAG234550.1337667326042652No Hit
TTGTAGGGGTTTTAGTTAAATGTCCT232850.13279720182009444No Hit
TCAACGATCTCATTCACGCAGCACGC230580.13150259306711348No Hit
AAACCTGTCCCAACGGAGATACGAGA230310.13134860876609813No Hit
AGCTCCTCAGGATCGACCCCAGCCTC227510.1297517345333463No Hit
AACACGTTCTACTTACTTCCCTCTCC227310.12963767208814975No Hit
GCCTCTATCCAAACACTCCCAGGGTA225300.1284913445139243No Hit
AGCTCCTGTTGTGGAGGATTTTCCCT225100.12837728206872775No Hit
ACACCCCTCCTCTGTCTGCCCCCCTC224660.12812634468929535No Hit
GGAACTTAGGTTCCTAGCCGGGCGAA224210.12786970418760307No Hit
TGACTAGGTGTTTGGAACAGAAAAGG223910.12769861051980824No Hit
ACACCAATCAGTATCGAAGGCCATTC223440.12743056377359632No Hit
ATCTACTCCCAAAACAAAATCTAATT223050.12720814200546302No Hit
AGAGGGTAATAATGACTTGTTGGTTG222040.1266321266572204No Hit
TTCTAAGTAGCATATTGTCCAATCCT221400.12626712683259142No Hit
GACTCAGGAGAAAAAGATTCTTATCT219820.12536603351553857No Hit
GGTGAAGTCTAACTTCGGGTCGTTGT219690.12529189292616083No Hit
GAGGTGTTCCCGGGGAGACAGGCCAC219170.12499533056864977No Hit
GGGTCTGTGTTAACAGACTAGAGGCT218260.12447634644300541No Hit
TTCTCAACAATAGCGGATTACTGACA217520.12405431539577814No Hit
GTCGGGTTGTCGGGTATAATACTAAG216840.12366650308210986No Hit
AATCCAGCACCTGGTGGTCTGTTGGG215680.1230049408999698No Hit
TGGGGTAAGATTTGCCGAGTTCCTTT213620.12183009771444525No Hit
GACAGAGGTACTTGACTCCGCTTCCT213480.12175025400280763No Hit
GATGTTCGCCGACCGTTGACTATTCT212860.12139666042269831No Hit
TATTCATTCCCCCATTAAGATTTTTT212690.12129970734428124No Hit
GAGCCCCTTCTACCCTCCTGATCTGG212140.12098603561999069No Hit
GACTACAAGAGGTTATACGAGCTCCA211930.12086627005253431No Hit
GGAACTTTCACTATTCGGGCGGGTGA211120.12040431714948824No Hit
GTCAGGCATGCATGTTGCTCACGGGT210300.11993666112418236No Hit
ACGGGTCCAATGGACGCCTGACAAAG209040.11921806771944402No Hit
AATCGGTTCGTAGGTTTTGGCTCACA208120.11869338047153985No Hit
CACACTCCACTCTTTCCCTACACGAC208000.1186249430044219Illumina Single End PCR Primer 1 (95% over 22bp)
TACACGAAGCTGCAAGGCACGTATTG206870.11798049018906136No Hit
ATGGGGTAGAAAATGTAGCCCATTTC205090.11696533442681198No Hit
AGAGTGGGTCTAGAGGCGAGCAGCAG203660.11614978794365657No Hit
GGAACTTTCACTATTCTCTACGGCGC202650.11557377259541395No Hit
CTGGTCTAGCTGCAAGGGTGGACGCA199540.11380010157260745No Hit
CTTAAACTCCTACCTATCCTGTACAT199230.11362330478255278No Hit
CAGCATATATCCCCACAAAGATTTAT199190.11360049229351347No Hit
GTCACAAAGCCTCGTGATCGCCTGGG198380.1131385393904674No Hit
ACAGGCCCCCGTCAATTCATTTGAGT196520.1120777586501394No Hit
CTGAAACCAGGTCTCGCTTTTGTCTA196190.11188955561556507No Hit
CGAGCACAGGTGATTAACATAAGGGA195610.11155877452449506No Hit
GGAACTTTCACTATTCGGACTAGGCC195510.11150174330189677No Hit
CACCAGGTCCAGAGGACCTCGGCAGT194450.110897212342355No Hit
CGATTGACACCTCGGACGCCGGGACC193500.11035541572767134No Hit
GGAACTTAGGTTCCTAAGCGGGCGGC193470.11033830636089186No Hit
TATCCAACAACGCATAAGCGTCGCTG193050.11009877522597908No Hit
GCTACAGAGTGAGTAACCCTGAGCCA192850.10998471278078252No Hit
AGAGTGGGTAGCCTCGCACGTAGGTA191300.1091007288305092No Hit
CTTGGCTTCTCTTGGCTGTGACGGTT190520.10865588529424261No Hit
AGCCTACACAGGTTGCCACAGCTTCG189820.10825666673605465No Hit
ATGGGGGAAGGCGCTTTGTGAAGTAG189400.10801713560114187No Hit
CCTTACGCACCAGGCTTCGTTTAGGC189340.10798291686758292No Hit
CATCCTCTGTCATCCCCTTTTCCTCC189250.10793158876724446No Hit
CAAGGCCAGGTGCACAAATAAATCGG188790.10766924514329237No Hit
CATGACAAGCCTCGTGAAACTTCGCG187610.10699627671663269No Hit
GGCTCGATCCTTTACAACCATCAGCA187390.10687080802691647No Hit
GACGGCTGGCCATAGATTTAAGTTTT186890.10658565191392505No Hit
ACCCAAGTTATCCGAGAGGGCGCGAT185950.10604955842150124No Hit
ACTTCTACCACTTGCAATAAATTGCA184540.10524541818286548No Hit
TACTATAGAACGAATATTATGATGAT184470.10520549632704668No Hit
GGTGTTATCAAAGTAGCTTAGTGGGG184000.10493744958083477No Hit
GCCTCTATCCAAACACGATCCTTCCC183410.10460096536750493No Hit
ATCTCCCATCACTTCATGGGAGATAG182340.10399073128570333No Hit
ACGGGTCCAATGGACGTGAACTTATC181740.10364854395011364No Hit
CTATAGACTCCTACCTATCCTGTACA180840.10313526294672913No Hit
TCATTACGCTCGCACCCTCTGTATTA180280.10281588810017878No Hit
AGGGTTTGCTGAAGATGGCGGTATAT179600.10242807578651048No Hit
TAGCCTCGCCTTAGGGGCCGACTCAC179140.10216573216255838No Hit
GAGGTGAGAAGTGGTGGTGTCTGAGC178800.10197182600572421No Hit
CTGATAGCAGACTCGCTACGGGGGCA176170.10047190485138946No Hit
CAACCTCAGATATGCAGATGACACCA175670.10018674873839807No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)