FastQCFastQC Report
Wed 28 Aug 2024
HWTCTDMXY_n01_CITE-seq_test_TCells_300D28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTCTDMXY_n01_CITE-seq_test_TCells_300D28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25818527
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGGCTGCACCATCGGGATGTCCTG1222630.4735475420421932No Hit
CCTCTATATAAATGCGTAGGGGTTTT1014610.3929774924804967No Hit
ACGAGGAGTTCAGTTATATGTTTGGG775090.3002069018112459No Hit
AGCGGTCCAATTGGGTGTGAGGAGTT768850.29779003271565413No Hit
TGCTACCTTTGCACGGTTAGGGTACC652430.2526983820571948No Hit
GGGCTGTTAATTGTCAGTTCAGTGTT644660.24968891525066475No Hit
ATGAGGGTAATAATGACTTGTTGGTT632550.24499848500264945No Hit
TGGGGTTTTAGTTAAATGTCCTTTGA603370.23369652343063568No Hit
TACAGTGTTTTAATCTGACGCAGGCT601680.23304195471724626No Hit
AGAGTGGGTGTTGAGCTTGAACGCTT498030.1928963646919129No Hit
AGGGGTTTTAGTTAAATGTCCTTTGA454570.1760634911511412No Hit
TGGGCTGTTAATTGTCAGTTCAGTGT445700.17262797370276003No Hit
AGAGGGATATGAAGCACCGCCAGGTC412670.1598348348842674No Hit
GAGGTGATGTTTTTGGTAAACAGGCG409930.15877358146729287No Hit
ACACGACGCTCTTCCGATCTACACGA397140.1538197744588605No Hit
AGAGGGTAATAATGACTTGTTGGTTG396150.1534363288811945No Hit
TGGGATTTTTTAGGTAGTGGGTGTTG365770.1416695847907977No Hit
AGAGGTGATGTTTTTGGTAAACAGGC342230.13255210105518414No Hit
GTAGGCCTACTATGGGTGTTAAATTT340920.132044713472616No Hit
GTAGGCCTTATTTCTCTTGTCCTTTC337770.13082465936186058No Hit
ATGTGTGAGGAGTTCAGTTATATGTT335010.12975565956957963No Hit
ATCCACCTTCGACCCTTAAGTTTCAT326910.12661837757049424No Hit
GGGGTAGAAAATGTAGCCCATTTCTT324780.12579338860036438No Hit
TACAGTGAGGGTAATAATGACTTGTT318260.12326807025048331No Hit
TCTCTATATAAATGCGTAGGGGTTTT317010.12278392179383432No Hit
GGGGTTTTAGTTAAATGTCCTTTGAA314240.12171104881390019No Hit
ACGAGGAGAATGTTTTCATGTTACTT310180.12013853462670429No Hit
GACGTAGGCTTATGCGGAGGAGAATG305080.11816320892357648No Hit
GTGGGTGTTGAGCTTGAACGCTTTCT304840.11807025241989987No Hit
AGAGGAGTTCAGTTATATGTTTGGGA293830.11380587281373565No Hit
AGTCTTTACGTGGGTACTTGCGCTTA276180.10696969660585207No Hit
CTCATTACAGCTGCTGTGCTGCGCTC267730.10369685303890497No Hit
GTGGGTATAATGCTAAGTTGAGATGA267060.10343734946614111No Hit
AGAGTGGGTATAATGCTAAGTTGAGA266310.10314686039215172No Hit
GTGAGGAGTTCAGTTATATGTTTGGG261780.10139230638525583No Hit
CTACACCGTTGTCTTTCCCGTTAGAC260460.10088104561503451No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)