FastQCFastQC Report
Wed 28 Aug 2024
HWTCTDMXY_n01_CITE-seq_test_TCells_290D7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTCTDMXY_n01_CITE-seq_test_TCells_290D7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13315121
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTTGTAGCCTTCATCAGGGTTTG1348621.0128484750532871No Hit
AGTCTCCTCTATATAAATGCGTAGGG1076910.8087872427145048No Hit
TACTTGTTGGTTGATTGTAGATATTG1035860.7779576317781866No Hit
GACGTAGGCTTATGCGGAGGAGAATG749390.5628112579675393No Hit
ACACGACGCTCTTCCGATCTACACGA725690.5450119454415773No Hit
CCCTCCTCTATATAAATGCGTAGGGG700200.5258682966531059No Hit
ACACCAAGTGCTGTATACGGAACAGT595510.4472434009424323No Hit
AACTCAGATCACGTAGGACTTTAATC577970.4340704076215305No Hit
AACCATGGTCAAAGATATGCACCCGA560200.42072467835628385No Hit
GAATAGAGAAAAAACCTGAGGCTCAG487810.36635791743837703No Hit
GAATGAACAAGTCTACCTTAGGCAGG468890.3521485084514065No Hit
GCATGTACACATTCGAGCAAGCTGCT458780.344555637158686No Hit
GAACCTAAGATATGCAACAGGGTCAA455780.34230255962375405No Hit
AGAATAGCAATAACGACCTGGGGCAG447690.3362267605378877No Hit
TAAGAGACAAGTCTACATCCGCATGC446080.33501760892747423No Hit
CTCCTAGTCTTGGGTATTGTAACGAT435350.32695910161086783No Hit
CCCTCCTGCCCCCCATCTTTCAGGTT424280.3186452455069691No Hit
TACTTGTCTCCTCTATATAAATGCGT414660.3114203768782875No Hit
GTTAAGCTACCTACTTCTTTTGCAAC410480.30828108884628236No Hit
AAAGATGGGAAACAATCCGAAAGTCC403050.30270096681810105No Hit
GATTGGCCTTTCACCCCCAGCCACAA401320.3014016921062903No Hit
TTCCCAGTCATGAATCACAAAGTGGT399540.3000648661022307No Hit
AAATGCCTCGGTCTAACTGGCCTTTC393040.29518319810987825No Hit
GAATAAGTCTTGTACTGACTTCGTAG385610.2896030760816969No Hit
AGCGATAGAGCCCAATAGCGATAGGT383470.28799588077344546No Hit
CAGTCCTCCAGTTAGTGTTACCCAAC381040.2861708879701506No Hit
GAAATGACATTAACCGAGAAGCGTAG379010.2846463055048467No Hit
GCACATACAACACCTAACTCATCCGG376980.2831217230395428No Hit
CGATGGCAGAAGGTTTTTGCTCGGGG368050.2764150622438955No Hit
CTGTGTTGGGTTGACAGTGAGGGTAA366730.27542370812852546No Hit
ATGCGATAAGAGTGATGTCATCTGCA357960.26883721146807454No Hit
CCCAGTTAAGACTCGGTTTCCCTTCG352930.2650595514678387No Hit
TGACTTTCACGGATAGGAGGTATGGG337220.25326093544324535No Hit
CTGTGCTGTGCGATAGTGAGTCCCAG334580.25127822721250526No Hit
AGCCAAGATGCGACTTCCGAACTGTT326130.24493205882244706No Hit
TTTGGTAAACAGGCGGGGTAAGATTT324900.244008297033125No Hit
GGCGGTGTGTACGCGCTTCAGGGCCC324340.24358772255993769No Hit
AACTCAGTTTACTGCTAAATCCACCT323610.24303947369310425No Hit
AGTCCTTCATGCATGTCTCAATGGGT323520.24297188136705627No Hit
TAAGTGCCACGGACAATTAATGTCGG320510.24071129357367463No Hit
CTACATTAGGCATGGTTGGCTTCTCT318150.2389388725795282No Hit
CAGTCCTTGCGTAGAGATCTGGAGGA317920.2387661366351834No Hit
CTTTGCGTCACGACTAGGATTGGTCA311910.23425247130686982No Hit
GCTGCATATAACTGTACCACTGCCGA311230.23374177373228525No Hit
TTTCCTCTGAACTAGATCATGCTCCT309090.23213457842403384No Hit
AAACCTGAGAACAACTCACGAACGAC300870.22596114597832045No Hit
CAAGGCCTTCAGTATCAAAGGCAGTT300580.22574334848327704No Hit
TTTGGTTGATTGTAGATATTGGGCTG297880.2237155787018383No Hit
ACGGCCAAGCGAGAAACTTGCCTTCG295120.22164274736970097No Hit
CTTAACTCAAGAGGCTCCATCAGCGG291540.2189540748446822No Hit
GCAGCAAAGAAGACCCTGAGTGCCTG287410.21585233810492596No Hit
ACTGATGAGGCATGGTTTTTGCGTAG282460.21213476017228833No Hit
GTCACAAGTTCGCGACCCAAATTTCG281420.21135369329351195No Hit
ACGGCCACAAACTGCTGTCGCTCAGG280190.21042993150418984No Hit
AGCGATGCTGTTTTGCATCTTAATGT279490.20990421341270574No Hit
TGGGCTGTTAATTGTCAGTTCAGTGT278110.20886779774663705No Hit
ACGCCAGTCCACTGGGTCAGATGTTT276940.20798909750801364No Hit
TTCGGTCAGGACTGGTATCTACACAC273920.20572099945618216No Hit
GACAGAGTCACGACTAATACCGTTCG273630.20550320196113878No Hit
TGGTTCCCAGACAAATTCAATTAATT273220.20519528136469806No Hit
TACTTGTCCTTTCGTACAGGGAGGAA273000.20503005567880309No Hit
TCAGCAATCCACTGGGGCGCGCCGAG272940.20498499412810442No Hit
CACAAGATCTTCAGTATCAAAGGCAG272180.20441421448592167No Hit
TACTGTGTCATGCCCGCCTCTTCACG265760.19959262856116738No Hit
TACCTTACAATCTACGTGCATACGAG263890.19818821023105987No Hit
CACATAGCAAGTACCTTGTGCTGATT262110.19685138422700027No Hit
GACGTTAGTTCCCGAGAGTCGGCTCG261260.19621301225876955No Hit
ATCCACCTATCCTATAAGCAAATTCA260530.19566476339193611No Hit
AAGGTTCCATACGCTATGCATCCACA256690.19278082414722328No Hit
CTTTGCGTCACGACTAGGGAGCGAGG256560.19268319078737625No Hit
TCGGTAACATGTAAGACCCATGGTTG256260.19245788303388306No Hit
CTGTGCTTACTTTGTAGCCTTCATCA253110.19009215162220455No Hit
GGCGGTATGTCCATCCCGGTCCTCTC250590.18819956649286176No Hit
CTGAAGTGTGTGGTTTCATCGCTTTC244740.18380606529974455No Hit
TTTATTCCAGTTCTAACCAAAAAAAA242260.18194352120420085No Hit
GACTACACAATCCAACGTACGCGGGC239170.17962285134322098No Hit
CAAGTTGCACTTAACGCTCGTTTGCT237510.17837614844055866No Hit
GCGCAACGCTTTACGCCCAGTAATTC234780.17632584788377065No Hit
GACAGAGGCTTATGCGGAGGAGAATG234240.1759202939274829No Hit
CTTTGGTAAACAGGCGGGGTAAGATT233000.17498902187971105No Hit
TACTTGTGTCATGCCCGCCTCTTCAC231670.17399015750589122No Hit
TTCTACACAGGTTTCATACTTCTGCG231450.17382493181999623No Hit
ACGCCACTAAACTCACAACAATTTCT228360.17150426195901636No Hit
TGTGCTCAGTAACGGCCTTAGGACCT228210.17139160808226978No Hit
TGCGCTTACTTTGTAGCCTTCATCAG227360.17075323611403906No Hit
ATGCGATAGACCGGATTTTAGACCCT227290.17070066430489064No Hit
TACGGTATCAGTGTTGGACCACCTTC227290.17070066430489064No Hit
ACGGGCTGTTAATTGTCAGTTCAGTG222700.16725345567644484No Hit
CTCCTAGTCTTGGGTATTTCCGCCCT221490.16634471440402232No Hit
CCACTACTCGAACTGTGTTATTTCGC221240.166156957942778No Hit
AGCTCCTCTATATAAATGCGTAGGGG220850.16586405786323685No Hit
TACTTTTTTTAACCTTTCCTTATGAG218620.16418927022893748No Hit
GGGCATCAGGCCGAATCTTTACAGGG218300.1639489419585447No Hit
AGCTCTCTCAGATAAGCGCGAGTTTT216000.1622215825150969No Hit
CTTTGCGTCACGACTACGGGGGTGCG215150.16158321054686622No Hit
CGAGCCACATCATCCCTCCGAGCTGC213910.16065193849909437No Hit
ATCGGTCAAACTGTCCCCAACGAGCT213610.16042663074560118No Hit
TCGGTAATCGTCTGAAAATTTCTGGG213280.16017879221675868No Hit
GTCATTTCAGGCTCACTACTGTGCAT212350.15948033818092977No Hit
TACTTTGTCATGCCCGCCTCTTCACG211850.15910482525844113No Hit
CACATAGCAAGTACCTATCCAAACGC210980.1584514327733109No Hit
CAATAGCCGATCTTGCTACCCAAGTA209740.15752016072553904No Hit
ACATCAGTCGTCTGAATCTAGTGGGC208440.15654382712706855No Hit
AAAGATGAGTGGTAATATTCCGCCAA205950.15467377277307506No Hit
TCTTGTCCTTTCGTACAGGGAGGAAT204360.15347964167956116No Hit
TACTTGTAGCCTTCATCAGGGTTTGC203500.15283375945288066No Hit
TTTACATCCTCTAGATAGTCAAGTTC202530.1521052643832527No Hit
CACATAGCAAGTACCTTGAGTTTGAG202470.15206020283255406No Hit
AACTGGTGATGCTAGAGGTGATGTTT200800.15080598967144196No Hit
AATCGGTTAACCTTGCTACAGAATAT198110.14878573014845303No Hit
AGCTCTCTCAGATAAGTACGTGTCAT196400.14750147595354185No Hit
GCGCCAACAGACAAATATACGGTCAG194970.1464275089952243No Hit
AGTGGGAAGAAGGTTTGCGTACGGGC194910.14638244744452567No Hit
GGCGACTTCATCGATGCGGGCACCTC193300.1451732958341122No Hit
ATTACTTATGTCAGCATTCGCACTTC193170.14507566247426515No Hit
CTAGAGTAGAAGGTTTGGTCCTGTTG192770.14477525213627424No Hit
CACACTCCACTCTTTCCCTACACGAC191310.14367875440260738Illumina Single End PCR Primer 1 (95% over 22bp)
TACGGTAGTGAGTGACAATCTAGTAA191280.14365622362725808No Hit
GCACGAAAGGTGTAATGATTTGGGCA190630.14316805682802283No Hit
AGCTCTCAGTTGACACAAAATAGACT189710.1424771130506437No Hit
TCCTAGAATAGGATTGCGCTGTTATC187590.14088493825929183No Hit
TGAGCATCAACTGCTATTCCACGCGA186940.14039677146005658No Hit
TAAACCGGTTGGACCCTCTGATCCCG186860.14033668939245839No Hit
TACGGGCTCGTCCAGGGCCCGTAGTG186320.13993113543617064No Hit
CAAGAGAGTTCCACCCTTCCACCAGG185690.13945798915383495No Hit
GACGTGCGTGCGATAGAAGTTTCATT185380.13922517114189198No Hit
GGCAATGCACTCCAGTATTCTTGCCT183880.13809863237442604No Hit
TACTTGTAGGAATGGAGGACCCGTCA182670.1371898911020035No Hit
TTCTACACAGGTTTCATGTATTTTTA180450.1355226137261539No Hit
ACTGGTGATGCTAGAGGTGATGTTTT177460.13327704644967175No Hit
GACTCTGCCGCTTACCGGATACCTGT175380.131714912692119No Hit
CTGTGCTTCATCGATGCTATATGTCG174460.13102396891473986No Hit
CAGAGAGGTAAATGTGTATTGGGCCT174090.13074608935209825No Hit
TGAGATTGTAAGAGATGCCATTGTGA171150.128538073367865No Hit
CAGCTCCTGACAAACACCATTCTGCT170370.12795227320878272No Hit
TACTTGTAGGAATGGAGTAAGAACGT170370.12795227320878272No Hit
TATTTGTAGGAATGGATATGCAACGG170120.1277645167475384No Hit
CGCGTTTAGACTCGGATAAAACTCAC169730.12747161666799722No Hit
CTTAACTATTCTTTGGTTGCTTTGTG169150.1270360216779104No Hit
GTCTCACGACGTTCTAAACCCAGCTC168480.1265328343617756No Hit
CACACCGTATACGTCCACTTTCGTGT168350.12643520100192857No Hit
CGACTTCCAAACCTACTGCGGTTCCG165850.1245576363894853No Hit
CTCGTCATCCGAGCCATGGATCCCTA165000.1239192644212546No Hit
AGAGGAGAATGGAGTCGTGACTCGTA163540.12282276668758774No Hit
ACAGAACCTAGCTCTTTCCACTACCC162350.12192904593206476No Hit
TTGACTTAGGGGCCGACTCACCCTGC162330.1219140254151652No Hit
GGTGTTACACCAGCACACTATGGTAG162280.12187647412291636No Hit
ATCGCTCTGGTCAGGTCACACTTCTG161860.12156104326802589No Hit
CTGCTGTTCAGTTCAGTCGCTCAGTC158520.1190526169458017No Hit
AGCTCTCTCAGATAATAGCGGGGGCT158040.11869212454021259No Hit
TTAGTTCGTCAGGACAGACCCAGCGG157390.11820395774097733No Hit
CGGCTAGCAAGTCTGTCTGGCCTCGG156160.11728019595165526No Hit
AAAGAGAAAGAGATAGGGAATCTACC155580.11684460096156844No Hit
AACTGGTCAGATCGGAGCCCTTACAC155420.11672443682637205No Hit
CTGGTTCCTCTGCCTTTTCTGAGTCC154260.1158532468461984No Hit
ACTTGTAGGAATGGACCCCGGAATGT153910.11559038780045634No Hit
ACGGGCTCATGACGGAGTCATTATGC153320.11514728255191972No Hit
CGCCAGTCCATGCTCACGCACCCGAT153270.11510973125967087No Hit
ACTTAGCCCAGTGGAGTCCCGAGAGG152550.11456899265128721No Hit
CCACTACTCGAACTGTCCCGAATCGT151600.11385551809855878No Hit
CTAGTGACAAAGGAAGGAAATATGGG151490.11377290525561128No Hit
TTCTTGGGCTGTTTCCCTCTCGACGA150400.11295428708458602No Hit
AGAGCGATCATTATCCGCCCGCGGCT150330.11290171527543759No Hit
CTGTGCTACCTTTGCACGGTTAGGGT148690.11167003288967484No Hit
TACGGGCTCCGCATAACTCTGGTTGA147440.1107312505834532No Hit
GTGGGTGTTGAGCTTGAACGCTTTCT146910.11033320688561524No Hit
AACTGGTTAAAAGTAAGAGACAGCTG146170.10977744776033203No Hit
GACCTGGACAGGAGGGTCGACTCCCC145940.10960471181598724No Hit
GCTCTCGAGAGCTAGATCGGAAAGCG145060.10894380907240722No Hit
ATCTACTCATCGGACCGCTTCGGCTC143870.10805008831688424No Hit
AGCGGTCCAGGTCTCGCATAAGCACA143780.10798249599083629No Hit
TTAGTTCCAATGAATGTATTGCTAAA143530.10779473952959195No Hit
CGTTCTGGTCAGAAGCCAACCGTAGA142830.10726902143810785No Hit
CTACATTTCCAATGGTGTGCGCGATG142640.10712632652756215No Hit
TAAACTGCCCAAAATAGGAAGAAAAA142530.10704371368461467No Hit
TGCACCTCAGGACGTACGCTGGCATC142200.10679587515577216No Hit
CAACCTCTCAACGGCCTCGATAATTT141920.1065855879191785No Hit
CACCAGGATTCTGCAGAGGTCAACAG141070.1059472159509478No Hit
CTGTGCTGTTATCCCTAGGGTAACTT140840.10577448000660301No Hit
TTGGAACAGCCCGAAAAGAACCTCGA140690.10566182612985642No Hit
AGCTCTCTCAGATAAGCTTAGATACA140620.105609254320708No Hit
TCAGACAATATGTTTGTTGTTGTTTA140120.10523374139821935No Hit
CTTTGGGCTGTTAATTGTCAGTTCAG140010.10515112855527187No Hit
TACTGTGTAGCCTTCATCAGGGTTTG139560.10481316692503209No Hit
TAAACCGTCTCTGTCGTCCCCTTCTC139390.10468549253138593No Hit
TACTTTACGTGGGTACTTGCGCTTAC138870.10429495909199772No Hit
CATGGCGTCTTACCGCCGTAAGAGAA137460.10323601265057973No Hit
GCATGCGGTTATCCGACGGCGGCCTG136910.10282294843584223No Hit
AACTGGTCAGATCGGACCGCGCTGGG136220.10230474060280788No Hit
ACTGAACCAGTAAGATCGTAAGACTA135610.10184661483737174No Hit
GTCCATTAGAAGAACTTTCCCTTCCC135070.101441060881084No Hit
GTCTTCGGAATAATCTCCCATATTGT134800.10123828390294014No Hit
ACGATGTTTTTGGTAAACAGGCGGGG133730.10043468624881442No Hit
GGTGCGTTCGGACAAGGGATCGTCGA133700.1004121554734651No Hit
AGAGTGGGTGTTGAGCTTGAACGCTT133280.10009672461857462No Hit
CTGTGCTGTGCGATAGCCGTTGCATT133200.10003664255097645No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)