FastQCFastQC Report
Wed 28 Aug 2024
HWTCTDMXY_n01_CITE-seq_test_TCells_253D28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTCTDMXY_n01_CITE-seq_test_TCells_253D28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5271898
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACGACGCTCTTCCGATCTACACGA1518732.880803080787982No Hit
ATAACTGCATGGGAACGTATAGCCCG370150.7021190470680578No Hit
GTAACTGGAATTGGAAACTCTCAAAA359430.6817848145013428No Hit
ACTGAGTTCCGAGCCATGGCACACTG298190.5656217172638773No Hit
CGGACACGTTGGTAAAGGATCGCTTA267450.5073125466388007No Hit
CTGCTTGTCCTATTGTCCTTGGATAA266680.5058519720980944No Hit
GTCTTCGGTTGGTAAACATTAGGTGA231720.4395380942499267No Hit
AGTTGGTTGATTGTAGATATTGGGCT215690.4091315879024973No Hit
TGGTTCCTGCTCGAGCTGTCACTCTC212090.4023029277121826No Hit
AAATTGCAGCACGCCAGGCCTCCCTG204270.38746956029877666No Hit
AGATTGCTGGGAGAAATATCAATAAC192700.36552300518712616No Hit
AACAACGCATAAGCGTCGCTGCCGCA192410.3649729186717952No Hit
CCACTACCACGTCAGCGCCTTAGTCC184110.349229063233014No Hit
CTCGAGGCAACTGCTAAATTGTCGGC182700.34655450465847404No Hit
CATATGGCACATCCGGATACGCGGGT180280.34196412753054023No Hit
ACGCACAATAAATCCGGACAACGCTC175470.3328402787762586No Hit
GCGGGTTTCCCTTCGGCTCCCCTATT173680.3294449171816298No Hit
AACTATGTGAGGCGATGGTTGACTTA167730.3181586593670818No Hit
TCCAATTAATGGGGACAATCTTATAA166510.31584450230258626No Hit
ACCGTAACAACTGCTACACAATGTGA165380.3137010617428486No Hit
GACTACAGTGAGGCTAGAGGCAATCT162790.3087882201059277No Hit
GACACAGTGGCACCCGTCCCGACCCT162360.30797257458319566No Hit
CACACTCCACTCTTTCCCTACACGAC161350.30605675602980176Illumina Single End PCR Primer 1 (95% over 22bp)
AAGCCTAACCCTAACCCTAACCCTAA161030.30544976401288493No Hit
CTAGCCTGTTGGTTTGCAGGCCATAG155810.2955482067369285No Hit
TGCTACCCATCTCTTTTAGAATTTTA155490.29494121472001167No Hit
TCTCTAACACCGATATTACAGATCGA155020.294049695195165No Hit
GTCCTCACTGTCCATCACCAATTCCC152990.2901990895878486No Hit
AACCATGACAGTGCTCTACCCCCGAA150050.2846223504324249No Hit
ATTACCCATAGAGGGATGTTAAAAGC148520.28172016985154114No Hit
TTCGAAGCAGTTAACCTGCACTCGCC142660.270604628541751No Hit
GACGGCTAGACTACAATTAAACTCTG140830.26713339294500765No Hit
TGCTACCAGGTGACCAATCTATCTTT138250.26223951980861543No Hit
TGACCTTCATCTCCTCCTGGGGAAAA138230.2622015828075581No Hit
CACACAAGTGTTTGTGCCGATGTGTG136850.25958392973460415No Hit
TGACAACGTAAGGGAATCGGGCGGGC135750.2574973946764524No Hit
TAATCTGAGCCACTTCCCCCTTCCTG135240.25653000114949115No Hit
ACCCATTACCGTCACTCACGCTTCGT134710.25552467062147255No Hit
CACATGCTAGTAAAGTAATGCTCAAA133880.25395028507759443No Hit
TCAGATGAGTGGAGAACAGCCAGTCG133860.2539123480765371No Hit
TGTTTTTGTTGACTGTATAGAGCTTT133370.2529828915506332No Hit
CGTTGGGCACGCATCGGCATGGCATC131700.24981515196234827No Hit
CAGCTACACAGGTTGCCACAGCTTCG130960.24841148292322804No Hit
ACCAGTATCAGTTGACTGGACGGACT130880.2482597349189988No Hit
CCTCTAGGAGATCTTCCCAACCCAAG130300.247159561888337No Hit
AACTCTTAGTGCGTGAAACCTCCTGC128280.24332792478154924No Hit
ACTGAGTTCCGTACAACTGCAGCTAC127670.24217084624930146No Hit
TATGCCCCATGATCCACCCGATGCTG127610.24205703524612956No Hit
GGTGTTAGTCGCCATGATGCCCCTTG126490.23993256318692052No Hit
TGCCAAAGTGAAAGAGAGTTCCACGC125780.23858579964938623No Hit
ATTACCCAATGGTTAGTGGCTTTTTC123300.23388161151828052No Hit
GGGAAGTTAAAGGCAATCTCTCCTAT122110.23162435995537092No Hit
ATTATCCAGTAGATGTTGATCAGCCT121600.23065696642840966No Hit
CTTAACTTCCGTACAAACACCGGGCA121290.23006894291202146No Hit
ACACTGAGTGGCAAACGTTCCGGCAA118580.22492847926875673No Hit
AACCGCTACACCTGGAATTCTACCCC118370.224530140757655No Hit
CTAGAGTCACTGTCGGCGGCCTATGG118100.2240179912433814No Hit
TCAATCTAGACAATACGCCGTCTATC117850.22354377873016512No Hit
TGCTGTGCATGAGTTTTTCATTGTGA116860.22166589717782856No Hit
CACCTTGCCCGAACTCAAGATCTTCA113610.21550113450601663No Hit
TACTCGCCAAGTACCTACGAAAGCAG112890.21413540246795365No Hit
GCGAGAATCTGTCTATGAGAGTGGGA112810.21398365446372447No Hit
TGTATTCTCAGTTTGGAGTAGAAGGG112150.21273173342883342No Hit
GTTCGGGGTTGTACACCGATTTTTCC111440.2113849698912991No Hit
GAATAATGGAAATAAAAACAAAAATA110990.21053138736750976No Hit
AAACCTGTCAAGGCTTAAAATCCAGC110110.20886215932098837No Hit
GACCTGGAAGTAAGAGATGAGATGGA109450.20761023828609732No Hit
TGACTTTTCAGGCAAGATGAATAGAT108790.20635831725120632No Hit
CAGCGACTCCTTGACCCCTTGGCTGC107830.2045373412004557No Hit
CTATTGATGGACATCTAGGTTCATCG105300.1997383105667067No Hit
GTCCTCAAGCATGCCTCACAGCACAC105270.19968140506512075No Hit
CTTACACCCTCTGTATTACCGCGGCT105160.1994727515593056No Hit
TGACTAGTCACTCTTAGCGGGAATCT104310.19786042901437018No Hit
ATCATGGAGCGATCCCAAGAAGAGGA103650.19660850797947912No Hit
CATCAGAGATGCGTCCACTCCGGTCC102790.19497721693401504No Hit
GGAGAAGATTCTGGAGAGTCCCTTGA101920.1933269573880223No Hit
AAGACCTTCATATCGGACCAGGCCTA101580.19268202837004814No Hit
CTACCCATCACGATGTGCCCGAGCCT101280.19211297335418856No Hit
GACTGGGGCATCGCTCTTGCTCTTTT100300.19025406030238065No Hit
TCCTCTGAGTTGTCGCGAAAGTAGGG98660.18714322621568172No Hit
TCTACCCAATAACGATTGCGGTTTTT98380.18661210820087945No Hit
TGCTACCACGTACCGACTTATGCAGT98270.18640345469506428No Hit
AATCGGTTCTACTTACTGTCATTACC96730.18348230561365186No Hit
CACTCCATCAGTTTGGAGGCATGCTT95880.18186998306871643No Hit
AACACGTAGCGCTTATGGACAAGCCA95400.18095949504334113No Hit
CATTATCCAGTATCTGCCATAGGCGG94790.17980241651109335No Hit
TCACAAGTCTCCCTGACCTGGCTTCA94250.17877811748254613No Hit
TAGGCATCACGCTTTCTACTCTCCAT91790.17411186635249773No Hit
CCTCTGACACCACCAGTGGATAAAGT89910.17054578825311112No Hit
TTCCTCAAGAAGGTTTCGATGTAAGT89820.17037507174835323No Hit
ATTCCGAGCAACGCTAGCCCCCTTCG89090.16899037120976165No Hit
TCTTCCTATAAGATGACTAAAACAAT88980.16878171770394648No Hit
GATCTAGTCACTTACTACACCCGGCC87610.16618303313152114No Hit
GGAATAAGTAGCGTAGTGGGGGGGCC87490.1659554111251773No Hit
CTGCTTCTTCTGCGGGTAACGTCAAT86890.16481730109345819No Hit
ATTCACAGCAAAATGGTCATAACATC86750.16455174208605705No Hit
AGTCTCCCACCCGCGATTCGACTAAT86100.16331878955169465No Hit
CATCAGATCCGAAGAGCCAAGTTCTC85430.16204790001627498No Hit
CTGAAAACTTCCGTGGATGTCAAGAC85420.16202893151574632No Hit
CTACACCCAGCCAATTGTGTTTCAGC84960.16115638049142833No Hit
ATAACGCCAAGAAAGGGACTTATTCA84640.16054938847451145No Hit
CAGCCCTGGGCACTGACGACTTTGCA80880.15341723227573825No Hit
TTAGTTCTCTCAAGCGCCTTGGTATA80450.15260158675300622No Hit
GCTCTAGGGGCATGACTCACAGTATG79460.15072370520066966No Hit
ACGCCGAGTTAAGACTCGGTTTCCCT79340.15049608319432584No Hit
GGTTTCTAGAAGCAGCAGGGCTGACA79260.15034433519009663No Hit
GCTGCTTAGTGGTAATAATCTCGCAT79170.15017361868533877No Hit
TTTCCTCCAGATCTCTACGCATTTCA78990.14983218567582302No Hit
TCTTTCCTCTGAGTGTCCACTACCTA78480.14886479214886178No Hit
ACCCGACATGAACCTAACCCGGTTAT78460.14882685514780444No Hit
ATGTGTGTCGTTACGATAACTCCGGG78300.148523359139346No Hit
GACTACACGACGCTCTTCCGATCTGA78030.14801120962507242Illumina Single End PCR Primer 1 (100% over 22bp)
CAGTCCTCACCACCAGCACAGCGGAT77790.14755596561238477No Hit
CGATGCAGGGATCGCTCCCATATAAG76410.14493831253943076No Hit
CAAGGCCCAGTTAACCACCGTTCGTG76360.14484347003678752No Hit
TACTTACTCTTTCCTCCTGTTCCGGT75510.1432311474918521No Hit
ACCTGACCACCTGTGTCGGTTTGGGG75500.14321217899132344No Hit
TGGCTGGTCATGCCCGCCTCTTCACG75460.14313630498920882No Hit
CTACATTCATAGAAACCCCTGGTACG75380.14298455698497958No Hit
AGATCTGTCCATGCTCTAAGCCAGCA75310.14285177748127903No Hit
ACACCCTTGCAATAAATTGCAAATCC75290.14281384048022175No Hit
TCGAGGCGTATATCCGGGCCACTTAC74560.14142913994163012No Hit
AGTTGGTGTAGCACGATCGTATCCCT74530.14137223444004418No Hit
ATTATCCGTGCCTGCACACCGGCTAT74040.14044277791414023No Hit
CTCGTACCACCGATATCTCTCCTCCG73710.1398168173966947No Hit
GAAGCAGTCGTTACGACCGACACAAG73390.13920982537977783No Hit
TGTCCCAGAATCAGGGTCTTTTCAAA72380.13729400682638396No Hit
ATAAGGACTCATACAATTAGGTTGGG71900.13638351880100869No Hit
ACAGGCATAGCTAGATCTTACTGTTC71650.13590930628779235No Hit
CTCTAATAGCGCTTATACCAGGCACC71550.13571962128250586No Hit
GACGTGCTCTTGGGTAACCCGCAGTC71370.13537818827299009No Hit
CCCAGTTGGCCCAGGAATATTTACCT71020.13471429075448726No Hit
ATTCTACGTAAGGGAAGCCAAGCCAC70710.13412626723809906No Hit
ACACCGGGTTTGGGCCCCGCATCGGC70280.133310621715367No Hit
ATCACGAGTTCCTTCAACATAGTTCT70130.13302609420743725No Hit
CTCCTAGAGAAGGACAAAAATTGCCG70090.13295022020532263No Hit
GCAGCCAGGGTGATGGTCTTTTCTTC69790.13238116518946308No Hit
CATGCGAAGTCGTCAGTGCCCAGGGC68870.1306360631408271No Hit
GGATTACGTCGTTGTACTCGTCCGCT68800.13050328363712652No Hit
GTTGGTGAAAAGAACCTGAAAAAAAA68210.12938414210593605No Hit
AACGTTGAGATTGCATCATCACAAAC67760.12853055958214668No Hit
CACCAACTGCAGCATGCCAGGCTTCC67270.12760110305624275No Hit
GTTGGTCATGCTGGCGTCTCAAGCCT66090.12536281999386178No Hit
CGGCTAGGTGACCAAGTAGTACCTTT65930.12505932398540337No Hit
ACACGACGCTCTTCCGATCTACACTA65570.12437645796637188No Hit
GCGCCAAGTTTGACTGCCCGCAATAG64990.12327628493571006No Hit
CGGCTAGAAGACAGGGAATAAGAAAC64790.12289691492513703No Hit
TACTGAGCTAAATGAAATTGTTAGAA64580.12249857641403533No Hit
TTTCAATAGAATTTATTTACATGTTC64480.1223088914087488No Hit
GATTCAGGAACCCCGCATCTTCACGG64310.12198642689976172No Hit
GATGAGGTCCTCAATTTGATCCTGCC63640.12071553736434204No Hit
ATTATCCCCAGCCTTCATGAGGCACA62610.11876178180989087No Hit
CAAGGCCAAAGTGGAAAGGTGGGGAG62530.11861003380566164No Hit
CAACAGATAAGTTGAATGCAAATCAA62220.11802201028927344No Hit
GATGCTAGAGGTGATGTTTTTGGTAA62210.11800304178874478No Hit
TTGCAAGATGAAGCCCTTTCCCGCTA62030.11766160877922903No Hit
ATCTGCCCTGTCAGCTCTCCGTTATT61790.11720636476654138No Hit
ACACTGATCTTACCTAGATACTACCC61710.11705461676231217No Hit
ATAAGAGGTCTGCAATGTGGAACCCT61700.11703564826178352No Hit
ACCGTAAAGTGCTAAGAGAAGTGGTG61170.11603031773376495No Hit
ACTGACACCAGTTGTGATGAAGGAAA60260.11430418418565762No Hit
AACTGGTACCTGCAGTAATGGCAGAT59090.11208486962380532No Hit
CATGCAACTCATTTGTGTTTGTGCGT58910.11174343661428958No Hit
CACAGGCAGAAACGAGTGATGTACGA58670.11128819260160193No Hit
AGTGTCATCTGTCAAGGCTTGCGGTA58590.11113644459737271No Hit
GCATGGGTGGGTGAGCTGGTCCCTCT58540.11104160209472946No Hit
GCTGCTGCTGCTGCTGCTGCTGCTGC58290.11056738958151315No Hit
CTTACCGAGGTGCTTTACAACCCCAG57770.10958102755402324No Hit
AACGCTGCCTACTTCTTTTGCAACCC57410.10889816153499177No Hit
TTCTCAACACGAAGCATGGGAGATCG57320.10872744503023389No Hit
ATCATGGTCAACACACCTCACTCGGG57160.10842394902177545No Hit
CACACTCTCGATCCCTACTACCTGCC57000.10812045301331703No Hit
TATTACCCAGGTCCACAATACGGCCT56940.10800664201014512No Hit
ACGTAAGTGCGAAACTTGGGCTTGTT56810.10776005150327263No Hit
TCAGCTCTCCTTTCTCCCCTGACAGC56750.10764624050010071No Hit
TGGCCAGCAAACTGTCCCGGGGTAAA56640.10743758699428554No Hit
TTCTATCCTAAAATGAATATGTATAA55840.10592010695199337No Hit
TACGGATGTTTAAGCCGGCCTGTGCC55720.10569248494564955No Hit
ACGTTGTCCAGGGCTGACGAATGATT55450.10518033543137596No Hit
ATTATCCGTTCCATGAACCGAGAGTA55080.10447850091181582No Hit
TCCCACCCTCTCTCCCTCCCACAGAG54740.10383357189384164No Hit
CTACCGCTTGCAATAAATTGCAAATC54460.10330245387903939No Hit
CCAGCCATCTCATCCTCTGTCGTCCC54090.10260061935947927No Hit
AACTTGTCAGTCCTTCGGTTCGATTG53760.10197465884203374No Hit
AACCGGGGCTTGGCAGTCAGGCTTGT53640.10174703683568992No Hit
ACTTACTTCACCTTATCGTGGTGGGT53260.10102623381560112No Hit
TGGCCAGCAAACTGTCCCAGGGTAAC53140.10079861180925731No Hit
CATGTCAGTTACTGCCCAGAGACCCG53120.1007606748082No Hit
CCTACACGACGCTCTTCCGATCTCCT52950.10043821029921292Illumina Single End PCR Primer 1 (100% over 23bp)
TCTCTAACCAGAACCATGACGAGTTT52940.10041924179868428No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)