FastQCFastQC Report
Wed 28 Aug 2024
HWTCTDMXY_n01_CITE-seq_test_TCells_029D7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTCTDMXY_n01_CITE-seq_test_TCells_029D7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9242217
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACGACGCTCTTCCGATCTACACGA1555431.682961999269223No Hit
CTAACTTCACCATGTACACGAGGCCA365310.3952623055701895No Hit
TCTCATACAGCTTCGGGACATTGGGC323120.349613085258656No Hit
ATCCCCACACACCTGCTCAGGTGCGC279000.3018756214012287No Hit
GTTCTCGTTGAGCTCATCTTAGGACA262460.2839794824120663No Hit
ATCCACCATTGTCCGAAGGACAATCC253170.27392778161343756No Hit
GACCAATGCGGTAGCCTCCCAGCCAA251450.2720667562772006No Hit
CACACTCCACTCTTTCCCTACACGAC249920.27041130932112933Illumina Single End PCR Primer 1 (95% over 22bp)
AGATTGCATCGAATTAAACCACATGC249020.26943751699402857No Hit
TGCACCTCAAGAGTCGGCTGCGTTTC233130.2522446724633278No Hit
GAGGTGATGTTTTTGGTAAACAGGCG228430.24715931253291284No Hit
GGATTACTGTGATATTGAGTGGTTTG227980.24667241636936246No Hit
ATCTACTGTTCCACAAGCACGACCGA220750.23884961800831989No Hit
CCTTAGCAATTACTTTGGAGATTATT216750.23452165211009435No Hit
GCTGCTGCTGCTGCTGCTGCTGCTGC212240.22964187055984508No Hit
GAGGTGATAGATTGGTCCAATTGGGT209360.22652573511312274No Hit
CAAGCACTAATTTACACTCACAACAC208050.22510832628145389No Hit
AACCGCGCAAAGGTAGCATAATCACT204320.2210724980813586No Hit
ACAGCCGCATACGCCGGGAGGGTTCA199600.21596549832145254No Hit
GATCACACGGACCACAGCCTTGTCTC190100.20568657931316695No Hit
ACCTTTAAGGATGGTCAGGTGCGCTC187600.202981600626776No Hit
GACAGAGGTTATCCGATTAGCGAGGC187470.20284094173508369No Hit
TAGTCATGAATCACAAAGTGGTAAGC186640.2019428888112019No Hit
CAGTCCTGAATCACAAAGTGGTAAGC185010.20017924270767498No Hit
GGGACCTAACCTGACTAGAAAAGCTA184420.19954086773768676No Hit
GAATGAAAGCTTCCCATCACCGTTTG184380.1994975880787045No Hit
TGATTCCAGTTTATGCTTCATCCAGT184360.19947594824921336No Hit
ACCATAAAAATTGTTGCCGCGGCTTC180350.1951371624362423No Hit
GCTTCCAGTCTCCACTATCCGCACGT179790.19453124721049073No Hit
GCCCCATACACCGGAATAGCTCCTGG178080.19268104178899934No Hit
ACCTATGTCGGTTTGCGGTACGGGTA177800.19237808417612356No Hit
AAGACCCTGTTCTTGGGTGGGTGTGG176700.19118789355411153No Hit
CAAGCCACAACCCTCAAGTTTCACAC176150.19059279824310554No Hit
GTAGTCAAGGATGGTCAAACCGGACA171590.18565891711912846No Hit
TACCTATTCACCCGAGGGAGTCACCG171040.18506382180812245No Hit
CCTACACGTCTCCACTGCGCAACGAG168180.1819693261908912No Hit
TGACTTTTCATCTGCCCTCTGTAGGC167780.18153652960106867No Hit
AGCTCCTCAGCGTCAGTGACGGCCCA166950.18063847667718688No Hit
AAGACCTCAATAGCAATAACAGTTAA166020.17963222460584946No Hit
CAGATCAAGCGGATCAACAGTCGCTT165990.17959976486161275No Hit
GAGGTGATGCTAGAGGTGATGTTTTT165610.17918860810128134No Hit
TGCCCTACAGCCACCACTCCATACAG165530.17910204878331681No Hit
GATGAGGAGTTTTATAGCCCTGGTAG163870.1773059429355532No Hit
TTCTCCTCGCCTGACCTGTGCTGTGC163450.17685150651623954No Hit
CTCCAGTAATTCCGATTAACGCTTGC157280.17017561911822673No Hit
CATTCGCGACATGTCAAGTCCTGGTA157110.16999168056755215No Hit
AGTGGGATTTTTTAGGTAGTGGGTGT156950.16981856193162312No Hit
AGATTGCCATGGCCCAACCTCTTGGC155290.16802245608385952No Hit
TGCTACCTACTTCTTTTGCAACCCAC153760.16636700912778826No Hit
GACAGAGGTTATCCGATCAGAGCAGT153170.16572863415780004No Hit
CCTTCCCTCACGGTACTCATCCACTA152050.16451680370629687No Hit
CCCTCCTAGTTGACCCCGGCAGTCTC151290.16369449018563403No Hit
ACTGAGTTCACCCGAGCAAGCTGCGT148760.1609570517550064No Hit
TACCTACTTCTTTTGCAACCCACTCC148620.1608055729485685No Hit
GAACTCAGTCAAGCGCAGTAGCCGTT147560.15965866198553877No Hit
GCAAACTCATCGACGCCGCTAACTCA147410.1594963632643553No Hit
CACATAGCAACTGCTACCGATACATG147000.1590527467597872No Hit
ACACCCCTTTGTCAACCAGCGCTGCT145330.15724582099727805No Hit
AAGCCCCCTAGACCATCCAGTGCTCT144520.15636940790288736No Hit
GGAATAAGTGAGGGTTTGCACGTCTT144340.15617464943746723No Hit
GGAGCAATCAAACGGGTCGGCATTGG144120.15593661131306483No Hit
TGTTTCTGGGTATGCACGCGCGGCAC143780.15556873421171566No Hit
GCACATGGTTTCAGGTACTATTTCAC143380.1551359376218931No Hit
TACCTATTCTACGAGTACCCAAGTGT142770.15447592282241374No Hit
AATCGGTTCTTGTCATCTTTCAGCTT140580.15210636149313525No Hit
CGTTGGGTCTCAACTTCGTCTGCTCG140150.15164110515907603No Hit
TACCTATCAACTTGACGGCAGCTCCT139590.15103518993332443No Hit
AGTGAGGAGTTCAGTTATATGTTTGG138820.15020205649791601No Hit
ATCATGGTGTGACGGGCGGTGTGTAC138770.15014795692418823No Hit
TGATTTCTCTGAGGGACAATATGGAT138270.14960696118691003No Hit
TCTCATAGCTTTCCTATGTGGCTGAT138050.14936892306250762No Hit
ATCGAGTTCTTCGGTCAGGGTTATTG137550.14882792732522943No Hit
TTGACTTCTATTAACATCAGAGTTTT137230.1484816900533714No Hit
GTACTTTTCCAATGGTGCCTATGACC135860.14699936173322917No Hit
CACAGTAGTGATAAACTGTCAGGAAG134810.14586327068494498No Hit
ATGCGATGATTATGGTAGCGGAGGTG134680.14572261179325263No Hit
CCAATCCCTCCAATCCACTATCGGTC134400.14541965418037686No Hit
GACCTGGCTTATGCGGAGGAGAATGT134150.14514915631173778No Hit
AGTGGGAAGCCAACAGCTGCTCATAG132850.14374256739481447No Hit
GGCAATTTCGTTACAGCCGTGCCGCC132200.14303927293635282No Hit
CATCAGAGACTCTTTAGTTCTTCTTC130850.14157858444570173No Hit
TGCCCATAGGAGCGAGTCGAGACCCT130600.14130808657706265No Hit
CAGGTGCCAAACCTCCCCGTCGATGT129050.13963099979150023No Hit
TTCTCAAGTAGCGCTCTAATCTCTTC128350.1388736057593108No Hit
TTATGCTCGTTTTATGCTGGGCCCGT128340.1388627858445652No Hit
TATTGCCCAACAAGAAGACCCAGTAA127690.1381594913861036No Hit
TTCGGTTCTCACCAGCTGCACTCACA127660.13812703164186688No Hit
TCGAGGCAGTCCTCCTCCACGTCGCA125380.13566009107987836No Hit
AGATTCAACGCGCGCGGCGGATAGAG123860.13401546403855266No Hit
GGACATTTCCTAATTGCAGGAGAACC123230.13333380940958212No Hit
GAAACTCCAGTACTTTGACCACCTCA122690.13274953401332168No Hit
GGAGCAATCAAACGGGCCACCAGGGT120890.1308019493591202No Hit
AAGACCTCAATAGCAATAGACCGGTC120720.13061801080844565No Hit
CGGAGCTCAACTGTCCACGTTCAGCT120350.13021767396285977No Hit
TTGGCTCATCGGACCCGTAATGTTTT120270.13013111464489527No Hit
TGCACCTTCGCCAGCACCGACTAGAG120230.130087834985913No Hit
TGCGGGTGTGGGTATAATACTAAGTT118920.12867042615424415No Hit
CCGTACTAGTGTACGGACCACACACA118440.12815107024645708No Hit
GTCACAACAAAGCAATGCTAGCTATC116820.12639824405767577No Hit
CGTAGCGCAACTTGACGGGTGTAGTG116630.12619266567751006No Hit
GTGAAGGTCTTGTCATATCGCAATGA115420.12488345599329684No Hit
AAAGTAGAGACTGTAAGCCAGGATGT114510.12389884375145055No Hit
AACCATGTGCAGCTGCATCGGGCAGG113700.12302243065705988No Hit
TGCGTGGAATCACTTGACCAGTGAGC112430.12164830148437328No Hit
CAGCTAACAGGAACGTCGAGAGGACC111510.12065286932778142No Hit
CACACCTATAAGGGCCATGAGGACTT111090.12019843290846774No Hit
CACACTCAGTTGAGATCGTAGAGTTA110880.1199712146988109No Hit
GAGAAACCGTGACTTTCTTATATGGG110360.11940857913204157No Hit
TAGCCGGTCCTCAATTCAGAATGGTC109700.11869446475883437No Hit
CTACGTCTCACAGTACACATGACCAC109250.11820756859528402No Hit
GGATTACAGAGGTAGACCTGGGCCCA108780.11769903260224251No Hit
CCTTTCTTCAAAAGGCACGCCATCAC108380.11726623601241996No Hit
ACTGAGTTTCAACCTTGCGGTCGTAC107800.11663868095717726No Hit
ATTGGACAGAGACTTAGTGTGGCCTG107540.11635736317379261No Hit
CTTAACTTCTTGAGACATCCTATTCG107110.11589210683973336No Hit
GGTGAAGTCTTAGCATGTACTGCTCG106950.11571898820380434No Hit
CTGCTCACACCTGTCACATACATGAC106170.11487503485365037No Hit
ATCCGAAGTTATGTGCCGAAGACGGA106050.1147451958767036No Hit
TTCTCAAGGAGTCACTGTCCTTTGCT105090.11370648406112949No Hit
TTAGCTACCGGGCAGTGCCATTGGCA104800.11339270653350814No Hit
ATTCTCGCTAGAGTGCCCAACTGAAT103390.11186709855438365No Hit
GTCACGGCACAGATTCCTTTGTCGGC102570.11097986554524741No Hit
GAATAAGAGCAACGGTTTTCAGCGCA102560.11096904563050185No Hit
ATTCTACCAAAGGCGTGCCACCCTAA101680.11001689313289226No Hit
TTAGTTCTCATGTCTTGGTTAGTCCG100710.10896736140257257No Hit
AACAAACCCACAGGTCCTAAACTACC100480.1087185033634246No Hit
CAAGGCCTTTCACCCCTACCCACAGC100470.10870768344867904No Hit
GACTACACAGTAAGCGACAAGCGACC100280.10850210506851332No Hit
AGTCCTTTCGTACAGGGAGGAATTTG100170.10838308600631212No Hit
ACCAGTATCTCTGTCGCGAGGTCGGC99750.10792864958699844No Hit
ATTACTTTAAGTTTCAGCCTTGCGAC98690.1067817386239687No Hit
CGATGTAAGAGACTTAGGTAGGCTTT98430.10650042084058403No Hit
GAATGGAGCTGATGTAATAGAAATGG97880.10590532552957803No Hit
GACAGAGGTTATCCGACAAACCAGCC97830.1058512259558502No Hit
ACGGAGATCCACTGGGGAACGCAGTA96780.10471513490756601No Hit
GGAGCAATCAAACGGGACTTTTAACC96120.1040010205343588No Hit
GCGACCAGTACGACCCCAACCCGACC96050.10392528113113986No Hit
GAGCAATCAAACGGGGGTTAGTAAGT95210.10301640829251249No Hit
GCGTTAAGTGGAATAGAGATTCAACT95090.10288656931556574No Hit
TCAGCAACACTTAACGGATACGTCTC94840.10261607144692664No Hit
TTTCCTCGCTGATTCTGCCAGGCCCG94370.10210753545388514No Hit
GGGCTGTTTCCCTCTCGACGACGAAG93460.10112292321203885No Hit
AGATTGCAGAGCTTCTTAAGGGCGCT93370.10102554397932878No Hit
CAATCCTCAGCTCGGCCTGTGTACGT93190.10083078551390862No Hit
GACGGCTCAAGTCTACGGTAATCTCG92970.10059274738950623No Hit
TTGTAGGGGTTTTAGTTAAATGTCCT92890.10050618807154171No Hit
AAGCCGCCAGCTGCTGGGAAACACGC92460.10004093173748246No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)