FastQCFastQC Report
Wed 28 Aug 2024
HWTCTDMXY_n01_CITE-seq_test_GEX_450D7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTCTDMXY_n01_CITE-seq_test_GEX_450D7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227667503
Sequences flagged as poor quality0
Sequence length26
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAAAAAAAAAAAAAAAAAAAAAAA12913680.5672166571792198No Hit
GCCAAATAAAAAAAAAAAAAAAAAAA11722540.5148973764604429No Hit
CTCGAAAAAAAAAAAAAAAAAAAAAA8470970.37207637841927754No Hit
CAAGAAAAAAAAAAAAAAAAAAAAAA6374810.28000526715488244No Hit
TGCCAAAAAAAAAAAAAAAAAAAAAA6143700.2698540599358179No Hit
GCAAATAAAAAAAAAAAAAAAAAAAA4199780.18446989336023067No Hit
CACAAACAAAAAAAAAAAAAAAAAAA3965280.17416978478478767No Hit
CCTAAAGAAAAAAAAAAAAAAAAAAA3827890.16813510709958462No Hit
ACACGACGCTCTTCCGATCTACACGA3821640.16786058394991926No Hit
CACAAAAAAAAAAAAAAAAAAAAAAA2953460.12972690265768846No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAA2744110.12053147523649872No Hit
GCCAAAAAAAAAAAAAAAAAAAAAAA2620710.11511129016950654No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAA2612500.11475067656010617No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)