FastQCFastQC Report
Wed 28 Aug 2024
HWTCTDMXY_n01_CITE-seq_test_GEX_450D0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTCTDMXY_n01_CITE-seq_test_GEX_450D0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180995556
Sequences flagged as poor quality0
Sequence length26
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAAAAAAAAAAAAAAAAAAAAAAA4713760.26043512361154325No Hit
GCCAAATAAAAAAAAAAAAAAAAAAA4146400.22908849762035044No Hit
CAAGAAAAAAAAAAAAAAAAAAAAAA3640340.20112869511558618No Hit
TGCCAAAAAAAAAAAAAAAAAAAAAA2534350.14002277492382187No Hit
CTCGAAAAAAAAAAAAAAAAAAAAAA2267930.1253030765020551No Hit

[OK]Adapter Content

Adapter graph