FastQCFastQC Report
Wed 28 Aug 2024
HWTCTDMXY_n01_CITE-seq_test_GEX_410D3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTCTDMXY_n01_CITE-seq_test_GEX_410D3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244892387
Sequences flagged as poor quality0
Sequence length26
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAAATAAAAAAAAAAAAAAAAAAA13901970.5676766913950657No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAA10403110.42480332391876274No Hit
CAAGAAAAAAAAAAAAAAAAAAAAAA7611580.31081325529323217No Hit
TGCCAAAAAAAAAAAAAAAAAAAAAA7149060.2919265922300802No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAA7109190.29029853018664886No Hit
GCAAATAAAAAAAAAAAAAAAAAAAA6512550.26593517584521725No Hit
CACAAACAAAAAAAAAAAAAAAAAAA4593280.18756320097447537No Hit
CCTAAAGAAAAAAAAAAAAAAAAAAA4417840.18039923797222818No Hit
ACACGACGCTCTTCCGATCTACACGA3834130.1565638706441291No Hit
CTCGAAAAAAAAAAAAAAAAAAAAAA3502930.14303956292442851No Hit
CACAAAAAAAAAAAAAAAAAAAAAAA3429080.1400239526433298No Hit
GCCAAAAAAAAAAAAAAAAAAAAAAA3328140.13590214219276647No Hit
GCAAACTAAAAAAAAAAAAAAAAAAA3274780.13372322594903696No Hit
GGCTGTTAATTGTCAGTTCAGTGTTT2757990.11262048746333629No Hit
ATCCGAAAAAAAAAAAAAAAAAAAAA2534500.10349443815090911No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)