FastQCFastQC Report
Wed 28 Aug 2024
HWTCTDMXY_n01_CITE-seq_test_GEX_387D0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTCTDMXY_n01_CITE-seq_test_GEX_387D0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185558873
Sequences flagged as poor quality0
Sequence length26
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAAAAAAAAAAAAAAAAAAAAAAA8936350.4815910904998868No Hit
GCCAAATAAAAAAAAAAAAAAAAAAA8643230.46579448669102447No Hit
TGCCAAAAAAAAAAAAAAAAAAAAAA4822670.2598997246550425No Hit
CAAGAAAAAAAAAAAAAAAAAAAAAA4581660.2469113939919219No Hit
CACAAACAAAAAAAAAAAAAAAAAAA4240060.228502142282358No Hit
GCAAATAAAAAAAAAAAAAAAAAAAA3633350.19580578073461352No Hit
CCTAAAGAAAAAAAAAAAAAAAAAAA3489130.18803358436004297No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAA3204050.17267026621788117No Hit
CTCGAAAAAAAAAAAAAAAAAAAAAA2510600.1352993774649623No Hit
GTCTTCGCAAAAAAAAAAAAAAAAAA2475020.1333819267160563No Hit
CACAAAAAAAAAAAAAAAAAAAAAAA2295500.12370736914316138No Hit
ACACGACGCTCTTCCGATCTACACGA2240470.12074173354135427No Hit
GCCAAAAAAAAAAAAAAAAAAAAAAA2079870.11208679845775954No Hit
ATCCGAAAAAAAAAAAAAAAAAAAAA1989720.10722850208300198No Hit
GCAAACTAAAAAAAAAAAAAAAAAAA1942480.10468267933487611No Hit
CTGAAACAAAAAAAAAAAAAAAAAAA1937980.1044401687005288No Hit
CTGAAAAAAAAAAAAAAAAAAAAAAA1905950.1027140318964968No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)