FastQCFastQC Report
Wed 28 Aug 2024
HWTCTDMXY_n01_CITE-seq_test_GEX_300D28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTCTDMXY_n01_CITE-seq_test_GEX_300D28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152231300
Sequences flagged as poor quality0
Sequence length26
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAAATAAAAAAAAAAAAAAAAAAA6174230.4055821634578434No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAA4147860.2724709044723391No Hit
TGCCAAAAAAAAAAAAAAAAAAAAAA3966340.26054694402530887No Hit
CTCGAAAAAAAAAAAAAAAAAAAAAA3155670.20729442631048936No Hit
CAAGAAAAAAAAAAAAAAAAAAAAAA2292980.15062474011586316No Hit
GCAAATAAAAAAAAAAAAAAAAAAAA2288910.15035738379689328No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAA2169020.1424818680521023No Hit
CCTAAAGAAAAAAAAAAAAAAAAAAA2072490.13614085933707457No Hit
GCCAAAAAAAAAAAAAAAAAAAAAAA1985520.1304278423688164No Hit
CACAAACAAAAAAAAAAAAAAAAAAA1969700.12938863426903666No Hit
CACAAAAAAAAAAAAAAAAAAAAAAA1829260.12016319902674417No Hit
CTGAAAAAAAAAAAAAAAAAAAAAAA1586590.1042223248438396No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)