FastQCFastQC Report
Wed 28 Aug 2024
HWTCTDMXY_n01_CITE-seq_test_GEX_290D7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTCTDMXY_n01_CITE-seq_test_GEX_290D7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190776616
Sequences flagged as poor quality0
Sequence length26
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAAATAAAAAAAAAAAAAAAAAAA9783040.5128007931538109No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAA8958670.46958952243916524No Hit
CAAGAAAAAAAAAAAAAAAAAAAAAA5531530.28994800914174934No Hit
TGCCAAAAAAAAAAAAAAAAAAAAAA5309380.2783035002570755No Hit
CTCGAAAAAAAAAAAAAAAAAAAAAA3154550.16535307450888007No Hit
CACAAACAAAAAAAAAAAAAAAAAAA2959020.15510391483199387No Hit
GCAAATAAAAAAAAAAAAAAAAAAAA2787450.14611067427676777No Hit
ACACGACGCTCTTCCGATCTACACGA2708480.1419712780731995No Hit
CCTAAAGAAAAAAAAAAAAAAAAAAA2352450.12330913763561044No Hit
GCCAAAAAAAAAAAAAAAAAAAAAAA2323850.12181000212311135No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAA2322360.12173190030794968No Hit
CTGAAAAAAAAAAAAAAAAAAAAAAA2172180.11385986634756116No Hit
ATCATGGCAAAAAAAAAAAAAAAAAA2029360.10637362390367591No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAA1994460.10454425923982213No Hit
CTGAAACAAAAAAAAAAAAAAAAAAA1970080.10326632484140509No Hit
ATCCGAAAAAAAAAAAAAAAAAAAAA1934540.10140341308915973No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)