FastQCFastQC Report
Wed 28 Aug 2024
HWTCTDMXY_n01_CITE-seq_test_GEX_253D28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTCTDMXY_n01_CITE-seq_test_GEX_253D28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226578629
Sequences flagged as poor quality0
Sequence length26
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAAAAAAAAAAAAAAAAAAAAAAA14207260.6270344234451167No Hit
GCCAAATAAAAAAAAAAAAAAAAAAA9593910.42342519426225317No Hit
CAAGAAAAAAAAAAAAAAAAAAAAAA7437130.3282361638793392No Hit
TGCCAAAAAAAAAAAAAAAAAAAAAA4836020.21343672266637292No Hit
CTCGAAAAAAAAAAAAAAAAAAAAAA4644060.20496460855538146No Hit
ACACGACGCTCTTCCGATCTACACGA4609900.20345696416055195No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAA4254160.1877564542947252No Hit
CACAAACAAAAAAAAAAAAAAAAAAA4231610.18676121480106583No Hit
GCAAATAAAAAAAAAAAAAAAAAAAA3887160.17155898670390488No Hit
CACAAAAAAAAAAAAAAAAAAAAAAA3213460.14182537930353528No Hit
CCTAAAGAAAAAAAAAAAAAAAAAAA2708940.11955849551901032No Hit
GCCAAAAAAAAAAAAAAAAAAAAAAA2494940.11011365065678812No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)