FastQCFastQC Report
Wed 28 Aug 2024
HWTCTDMXY_n01_CITE-seq_test_GEX_029D7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTCTDMXY_n01_CITE-seq_test_GEX_029D7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129664180
Sequences flagged as poor quality0
Sequence length26
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAAAAAAAAAAAAAAAAAAAAAAA4907880.3785070017023977No Hit
GCCAAATAAAAAAAAAAAAAAAAAAA4802530.37038216722613754No Hit
TGCCAAAAAAAAAAAAAAAAAAAAAA3085850.23798785447145082No Hit
CAAGAAAAAAAAAAAAAAAAAAAAAA2618230.2019239237852736No Hit
ACACGACGCTCTTCCGATCTACACGA2278620.17573241893019337No Hit
GCAAATAAAAAAAAAAAAAAAAAAAA1938600.1495092939314466No Hit
CTCGAAAAAAAAAAAAAAAAAAAAAA1568500.12096633009980087No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAA1424190.1098368107522062No Hit
CACAAACAAAAAAAAAAAAAAAAAAA1313770.10132096620670411No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)