FastQCFastQC Report
Wed 28 Aug 2024
HWTCTDMXY_n01_CITE-seq_test_BCells_450D7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTCTDMXY_n01_CITE-seq_test_BCells_450D7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471496
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACGACGCTCTTCCGATCTACACGA65171.382196243446392No Hit
CGGACACGTTGGTAAAGGATCGCTTA9420.19978960585031474No Hit
ATAACTGCATGGGAACGTATAGCCCG8760.18579160798819078No Hit
GTCTTCGGTTGGTAAACATTAGGTGA8570.18176188133091267No Hit
AGTTGGTTGATTGTAGATATTGGGCT8520.18070142694741842No Hit
GTAACTGGAATTGGAAACTCTCAAAA7820.16585506557849908No Hit
ACTGAGTTCCGAGCCATGGCACACTG7440.15779561226394284No Hit
CTGCTTGTCCTATTGTCCTTGGATAA6530.1384953424843477No Hit
CACACTCCACTCTTTCCCTACACGAC6530.1384953424843477Illumina Single End PCR Primer 1 (95% over 22bp)
CTAGCCTGTTGGTTTGCAGGCCATAG6440.13658652459405807No Hit
AAATTGCAGCACGCCAGGCCTCCCTG5900.12513361725232028No Hit
CATATGGCACATCCGGATACGCGGGT5800.12301270848533179No Hit
GCGGGTTTCCCTTCGGCTCCCCTATT5790.12280061760863295No Hit
AACAACGCATAAGCGTCGCTGCCGCA5580.11834670919795715No Hit
ACGCACAATAAATCCGGACAACGCTC5290.11219607377369056No Hit
AACTATGTGAGGCGATGGTTGACTTA5170.10965098325330437No Hit
AGATTGCTGGGAGAAATATCAATAAC5170.10965098325330437No Hit
TGACAACGTAAGGGAATCGGGCGGGC5110.10837843799311128No Hit
GACACAGTGGCACCCGTCCCGACCCT4920.10434871133583316No Hit
CACACAAGTGTTTGTGCCGATGTGTG4870.10328825695233894No Hit
GTCCTCACTGTCCATCACCAATTCCC4730.10031898467855506No Hit

[OK]Adapter Content

Adapter graph