FastQCFastQC Report
Wed 28 Aug 2024
HWTCTDMXY_n01_CITE-seq_test_BCells_450D0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTCTDMXY_n01_CITE-seq_test_BCells_450D0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences963187
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACGACGCTCTTCCGATCTACACGA47800.4962691564566382No Hit
CGACCTTCCGATCTTTCTCAACAATA43380.450379832784288No Hit
AGTTGGTTGATTGTAGATATTGGGCT39780.413003913051152No Hit
GGACTTTAATCGTTGAACAAACGAAC27140.2817729059881415No Hit
GGTGAAGTCTTAGCATGTACTGCTCG24770.25716709216382694No Hit
CTTCTTCTGCGCGCAGCGGATAGGGA24570.25509065217865273No Hit
AACTTTCAGTGCTGCCAGGGGGCAAT23730.24636960424092103No Hit
GAATGAATCACATGCATTGACCGGGG23030.23910206429281125No Hit
GCTCCTAACCAACAAACTTAAAATAA22490.23349567633284088No Hit
ACTCCTAAGAAGGTTTTACCAATACG20940.2174032664477407No Hit
ATTGGACCAGAAGCACATACCTAGCT20340.21117394649221802No Hit
GGACTTGTTGGTTGATTGTAGATATT18760.1947700706093417No Hit
GTGCTTCTCCACGACGAAGATGAGGA18730.19445860461156556No Hit
AACACGTTCTACTTACTTCCCTCTCC18270.18968279264566487No Hit
GCTTCCAGAAGAACCACAAACAGAGC17930.18615284467086868No Hit
TTGCCGTGTTGGGTTGACAGTGAGGG17850.185322268676799No Hit
TTAGGCACAGCTGTATCGCTAACTGG17810.18490698067976416No Hit
TCACTCGCATCAACTCCACCACACAG17800.18480315868050545No Hit
TGTTCCGGCGAGCGGGCTTTTCACCC17720.18397258268643576No Hit
TCACAGATGCACAATGTGAGGCTTAG17620.18293436269384866No Hit
TGCTCTCGCGAGGTCGCTTCTCTTTG17430.18096174470793314No Hit
ATCTGCCTGATACTCGCCAGCTGAGC17090.17743179673313697No Hit
GGCATTAGTTAGCGGAACCCGTGTTT17070.17722415273461956No Hit
GCATACATCACTATTCTTAACTCTTG16850.1749400687509279No Hit
GGCTGGTTTCGTATTAAACAACATGC16720.17359038276056465No Hit
TACGGTAGTGTTGGGATATACTTCGG16640.17275980676649497No Hit
TTGCATGAAATGTTCCCTTGGTATCT16290.1691260367924401No Hit
GGATTACTCCGGTCTGAACTCAGATC16260.16881457079466397No Hit
ATCACGAGTACGACCCCTGGCTCGCA16210.1682954607983704No Hit
GTAGGCCTTATTTCTCTTGTCCTTTC15540.16133938684803678No Hit
TTGTAGGGGTTTTAGTTAAATGTCCT15500.16092409885100192No Hit
TAGACCACAGGCCAACAGCTGCCAAC15370.1595744128606387No Hit
GCATGCGCAGGGCATACCCCCCTTCA15050.15625210888435995No Hit
GGATGTTAGGATTCCGTTGGCCCGTA14870.15438331289770313No Hit
TCAACGAGTAAATACGCAGATCCACG14860.15427949089844442No Hit
TGGGAAGTCCAGTAGTGATCGGAAAG14800.15365655890289218No Hit
TGGTTCCCATCGGAAGCGCATATTCC14740.1530336269073399No Hit
ACCTTTACACGGTTAGGGTACCGCGG14670.15230687291252892No Hit
ACTTTCAACAAGAACCACGCAACGCT14580.15137247491920053No Hit
CTACATTAGTTACACAACCCCAAAGA14550.1510610089214244No Hit
CTAATGGTCGCCAGCAACACAGGGCT14420.14971132293106115No Hit
TTGCTATCCTCATTCTATTACTCTTT14310.14856928093921534No Hit
AAAGTAGCAGGTTTCAGCGCATAGTC14180.1472195949488521No Hit
AGCCTACACAGGTTGCCACAGCTTCG14170.14711577294959338No Hit
GGAACTTAGGTTCCTAGCCGGGCGAA14110.14649284095404114No Hit
TTCTCAACAATAGCGGGGTGAGACAA14040.14576608695923013No Hit
GGGACCTCCACGACGTCTTACCGACG13940.14472786696664303No Hit
TAACGAAAAGGTGGCGGGTTCAGATG13820.1434820029755385No Hit
GGCTGGTTGTTAAGCGTACACCGGCC13690.14213231698517526No Hit
CTGAAACCAGGTCTCGCTTTTGTCTA13670.14192467298665784No Hit
TGGGGTAAGATTTGCCGAGTTCCTTT13380.1389138350081552No Hit
GGAACTTTCACTATTCGGGCGGGTGA13020.13517624303484163No Hit
TTAACTCGTTGGAGGTCGCTTAGTGA12990.1348647770370655No Hit
CATCAGAGGTGGGCTTCACACTTAGA12900.13393037904373709No Hit
ACGGGCTAGGCATGCTCATAAGGAAA12800.13289215905114998No Hit
GACGCGTTCATGCATGATTGGTCTAA12690.13175011705930417No Hit
GGGTACCTCTGAAATCAGAGGTACCC12590.13071189706671704No Hit
CATCGGGAGACAAAGGCTCGTTTACG12560.13040043106894092No Hit
ACTGCTCGGAGGTTGGGTTCTGCTCC12450.1292583890770951No Hit
ACGGGCTGTTAATTGTCAGTTCAGTG12410.12884310108006025No Hit
AATTGTCAGTTCAGTGTTTTAATCTG12380.1285316350822841No Hit
ACATCAGCATGGAATACATCCAAACG12340.12811634708524927No Hit
CGGACACGTGCAACTTCTCGACTCGA12320.12790870308673186No Hit
AGGGATGTCTGGGCCACAATCAAATG12260.12728577109117958No Hit
CACCATTGGCTCCATTACCCCCACAA12240.12707812709266217No Hit
ATAACGCCATGAACCTAGACTCTCAG12190.1265590170963686No Hit
GCTGCGAGTGATGATAGCGAACACGG12030.12489786510822924No Hit
AGAGTGGGTAGCCTCGCACGTAGGTA11890.12344435711860728No Hit
AGAGGGTAATAATGACTTGTTGGTTG11840.12292524712231374No Hit
GGACTGTTAATTGTCAGTTCAGTGTT11780.12230231512676147No Hit
ACAGTGTTTAGAAAAGCCAGGCAGAA11640.12084880713713951No Hit
GTCGGGTTGTCGGGTATAATACTAAG11520.11960294314603498No Hit
CTTAAACTCCTACCTATCCTGTACAT11410.11846090115418917No Hit
GGGAATGTTCCTCTTTCAGTATCCTA11380.11814943515641305No Hit
TACTATACAGGTTGCCACAGCTTCGG11350.1178379691586369No Hit
AGGGGTTTTAGTTAAATGTCCTTTGA11290.11721503716308464No Hit
AATCGGTTCTGTCTATGCATTCCTGA11220.11648828316827367No Hit
GGTGCGTGCGGCCCAGAACATCTAAG11220.11648828316827367No Hit
GACTGCGCACAGACAGTTGTTTATCC11110.11534624117642783No Hit
AACGTTGCATCCCATCTCCACACAGG11070.11493095317939299No Hit
ACTGCTCCACGAAAGCAGCTTGTGCC10840.11254304719644265No Hit
GCTCCTAAGGATATACTCGTTGCATT10760.11171247120237296No Hit
GGAACTTAGGTTCCTAGGGTGTCTGT10740.11150482720385553No Hit
GGAACTTAGGTTCCTAAGGCGGCTGG10670.11077807320904456No Hit
GATGTTCGCCGACCGTTGACTATTCT10530.1093245652194226No Hit
GCCTCTAGGCTCGACGCCGGAATATA10530.1093245652194226No Hit
TGCGTGGTCCATTCTAGTGGGGCGTC10490.10890927722238776No Hit
GCCTCTATCCAAACACGATCCTTCCC10480.10880545522312904No Hit
GCATGTATCCGCATAAGGCTTATAAT10380.10776723523054195No Hit
GCATGCGAGATGTAACGGGTACTGGC10360.1075595912320245No Hit
TTCTCAACAATAGCGGCCGCACAGGA10230.10620990524166127No Hit
TGGCCAGTGAGAGTGAGTAGTAGAAT10210.10600226124314385No Hit
CACATTTTCTCACATTTAAGCGCTCC10210.10600226124314385No Hit
GCACAGGCCTGCGAGATCCTTTTCAA10180.10569079524536773No Hit
CTCGGAGTCAACTCTTAAGGGGAAAT10150.10537932924759158No Hit
AGGGAGTTCAAAGACAATGCATGGGT10030.10413346525648705No Hit
CACACCTCATCATAATCAATGCTGCT10010.10392582125796965No Hit
GACGCAGGCTTATGCGGAGGAGAATG9930.10309524526389996No Hit
ATGGGGGAAGGCGCTTTGTGAAGTAG9880.1025761352676064No Hit
ATGGGGTAGAAAATGTAGCCCATTTC9870.10247231326834767No Hit
TACTCATTCTAAGCCACGCCTCAGGA9850.10226466926983027No Hit
GGGTCTGTGTTAACAGACTAGAGGCT9820.10195320327205412No Hit
ATAGCGTATATGTGTCAATCCCAATC9790.101641737274278No Hit
GACTACAAGAGGTTATACGAGCTCCA9760.10133027127650186No Hit
TTCGAAGAGAGTACCGTGGACCGTTA9760.10133027127650186No Hit
AGAGTGGGTCTAGAGGCGAGCAGCAG9730.10101880527872574No Hit
CATCAAGTCTTCGGTCTCTGAAACGC9680.10049969528243217No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)