FastQCFastQC Report
Wed 28 Aug 2024
HWTCTDMXY_n01_CITE-seq_test_BCells_410D3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTCTDMXY_n01_CITE-seq_test_BCells_410D3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686441
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTTGTAGCCTTCATCAGGGTTTG41260.6010713229541942No Hit
TACTTGTTGGTTGATTGTAGATATTG33740.4915207570643362No Hit
ACACGACGCTCTTCCGATCTACACGA32540.4740392837840397No Hit
GACGTAGGCTTATGCGGAGGAGAATG32270.47010595229597296No Hit
AGTCTCCTCTATATAAATGCGTAGGG31350.4567034894477457No Hit
AACTCAGATCACGTAGGACTTTAATC28180.4105232641989625No Hit
CCCTCCTCTATATAAATGCGTAGGGG25940.37789118074240907No Hit
AACCATGGTCAAAGATATGCACCCGA15420.22463693165180984No Hit
ACACCAAGTGCTGTATACGGAACAGT14930.21749866339568877No Hit
CGATGGCAGAAGGTTTTTGCTCGGGG14560.21210854246759736No Hit
TACTTGTCTCCTCTATATAAATGCGT14390.20963200041955538No Hit
GTTAAGCTACCTACTTCTTTTGCAAC14340.208903605699543No Hit
TTCCCAGTCATGAATCACAAAGTGGT14330.20875792675554053No Hit
GAATGAACAAGTCTACCTTAGGCAGG14050.20467891632347138No Hit
GCACATACAACACCTAACTCATCCGG13320.194044353411291No Hit
TAAGAGACAAGTCTACATCCGCATGC13060.19025670086722676No Hit
CTGTGTTGGGTTGACAGTGAGGGTAA12570.1831184326111057No Hit
GGCGGTGTGTACGCGCTTCAGGGCCC12450.18137028528307603No Hit
CTGTGCTGTGCGATAGTGAGTCCCAG12230.1781653485150217No Hit
GATTGGCCTTTCACCCCCAGCCACAA12160.1771455959070044No Hit
AGAATAGCAATAACGACCTGGGGCAG12150.1769999169630019No Hit
CCCAGTTAAGACTCGGTTTCCCTTCG11950.1740863380829525No Hit
AGCCAAGATGCGACTTCCGAACTGTT11850.1726295486429278No Hit
AAATGCCTCGGTCTAACTGGCCTTTC11790.17175547497891297No Hit
ATCCACCTATCCTATAAGCAAATTCA11400.16607399616281662No Hit
CAGTCCTCCAGTTAGTGTTACCCAAC11300.1646172067227919No Hit
GAATAGAGAAAAAACCTGAGGCTCAG11250.16388881200277955No Hit
GAAATGACATTAACCGAGAAGCGTAG11200.1631604172827672No Hit
CAAGGCCTTCAGTATCAAAGGCAGTT10900.15879004896269308No Hit
CAGTCCTTGCGTAGAGATCTGGAGGA10880.15849869107468814No Hit
AACTCAGTTTACTGCTAAATCCACCT10770.15689622269066095No Hit
GACTACACAATCCAACGTACGCGGGC10710.15602214902664613No Hit
GCAGCAAAGAAGACCCTGAGTGCCTG10420.15179745965057448No Hit
AAACCTGAGAACAACTCACGAACGAC10340.15063202809855472No Hit
ATGCGATAAGAGTGATGTCATCTGCA10160.14800980710651024No Hit
GAACCTAAGATATGCAACAGGGTCAA9950.14495054928245837No Hit
GAATAAGTCTTGTACTGACTTCGTAG9900.14422215456244603No Hit
GTCACAAGTTCGCGACCCAAATTTCG9860.14363943878643612No Hit
CCCTCCTGCCCCCCATCTTTCAGGTT9830.1432024019544287No Hit
CTTTGCGTCACGACTAGGATTGGTCA9660.1407258599063867No Hit
AGCGATAGAGCCCAATAGCGATAGGT9650.14058018096238425No Hit
AGTCCTTCATGCATGTCTCAATGGGT9600.13985178624237188No Hit
TAAGTGCCACGGACAATTAATGTCGG9390.13679252841832001No Hit
GGCGGTATGTCCATCCCGGTCCTCTC9100.13256783904224834No Hit
TTTGGTAAACAGGCGGGGTAAGATTT9090.1324221600982459No Hit
CACACTCCACTCTTTCCCTACACGAC9030.13154808643423105Illumina Single End PCR Primer 1 (95% over 22bp)
GCATGTACACATTCGAGCAAGCTGCT8850.1289258654421866No Hit
TACTGTGTCATGCCCGCCTCTTCACG8840.1287801864981841No Hit
ACGCCAGTCCACTGGGTCAGATGTTT8820.12848882861017916No Hit
TGCGCTTACTTTGTAGCCTTCATCAG8810.12834314966617671No Hit
GCTGCATATAACTGTACCACTGCCGA8780.1279061128341693No Hit
TTCGGTCAGGACTGGTATCTACACAC8710.126886360226152No Hit
GACAGAGTCACGACTAATACCGTTCG8630.12572092867413223No Hit
ACTGATGAGGCATGGTTTTTGCGTAG8410.12251599190607786No Hit
TCAGCAATCCACTGGGGCGCGCCGAG8390.12222463401807293No Hit
TTTCCTCTGAACTAGATCATGCTCCT8340.12149623929806057No Hit
TGGGCTGTTAATTGTCAGTTCAGTGT8260.1203308077460408No Hit
AAAGATGGGAAACAATCCGAAAGTCC8240.12003944985803587No Hit
CTGTGCTACCTTTGCACGGTTAGGGT8220.11974809197003093No Hit
CACAAGATCTTCAGTATCAAAGGCAG8010.11668883414597904No Hit
TACGGTAGTGAGTGACAATCTAGTAA7940.11566908153796175No Hit
TACTTGTCCTTTCGTACAGGGAGGAA7930.11552340259395927No Hit
CTCCTAGTCTTGGGTATTGTAACGAT7900.11508636576195187No Hit
TGACTTTCACGGATAGGAGGTATGGG7810.11377525526592963No Hit
CTGTGCTTACTTTGTAGCCTTCATCA7810.11377525526592963No Hit
TACTTGTGTCATGCCCGCCTCTTCAC7800.11362957632192715No Hit
GCGCAACGCTTTACGCCCAGTAATTC7780.11333821843392221No Hit
GACGTTAGTTCCCGAGAGTCGGCTCG7770.11319253948991975No Hit
CGAGCCACATCATCCCTCCGAGCTGC7690.11202710793789998No Hit
CTACATTAGGCATGGTTGGCTTCTCT7650.11144439216189009No Hit
TTTACATCCTCTAGATAGTCAAGTTC7620.11100735532988269No Hit
ACGGCCACAAACTGCTGTCGCTCAGG7560.11013328166586786No Hit
CTGAAGTGTGTGGTTTCATCGCTTTC7550.10998760272186538No Hit
AGCTCCTCTATATAAATGCGTAGGGG7530.10969624483386045No Hit
TGGTTCCCAGACAAATTCAATTAATT7490.10911352905785057No Hit
CTTTGGTAAACAGGCGGGGTAAGATT7460.10867649222584316No Hit
CAGCTCCTGACAAACACCATTCTGCT7280.10605427123379868No Hit
ACGGCCAAGCGAGAAACTTGCCTTCG7240.1054715554577888No Hit
TACTTGTAGCCTTCATCAGGGTTTGC7220.10518019756978385No Hit
CCACTACTCGAACTGTGTTATTTCGC7210.10503451862578139No Hit
TCGGTAACATGTAAGACCCATGGTTG7180.10459748179377397No Hit
TTTATTCCAGTTCTAACCAAAAAAAA7080.10314069235374927No Hit
TTTGGTTGATTGTAGATATTGGGCTG7070.10299501340974679No Hit
AATCGGTTAACCTTGCTACAGAATAT7030.10241229763373691No Hit
CACATAGCAAGTACCTTGTGCTGATT7010.10212093974573198No Hit
TACCTTACAATCTACGTGCATACGAG6950.10124686608171715No Hit
AACTGGTGATGCTAGAGGTGATGTTT6940.10110118713771467No Hit
GCACGAAAGGTGTAATGATTTGGGCA6890.10037279241770232No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)