FastQCFastQC Report
Wed 28 Aug 2024
HWTCTDMXY_n01_CITE-seq_test_BCells_387D0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTCTDMXY_n01_CITE-seq_test_BCells_387D0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4440030
Sequences flagged as poor quality0
Sequence length26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGCGGGCTTATGCGGAGGAGAATG525561.1836856958173707No Hit
TAGTTGGTTGATTGTAGATATTGGGC194240.43747452156854794No Hit
AGTTGGTTGATTGTAGATATTGGGCT190470.42898358794873004No Hit
GGGTCTGTTAATTGTCAGTTCAGTGT186640.4203575201068461No Hit
CGTAGGACTTTAATCGTTGAACAAAC141760.3192771220014279No Hit
CCTCTATATAAATGCGTAGGGGTTTT118980.2679711623570111No Hit
GGGCTGTTAATTGTCAGTTCAGTGTT114720.25837663259032034No Hit
TAGTTGGGTTGACAGTGAGGGTAATA110740.2494127291932712No Hit
ACGCCGAGTTCCTTTTACTTTTTTTA105920.23855694668729716No Hit
GTCACGGTTAGGGTACCGCGGCCGTT78200.17612493609277416No Hit
CGTAGGGGTTTTAGTTAAATGTCCTT69000.1554043553759772No Hit
CATGGCGGTATATAGGCTGAGCAAGA65460.14743143627407923No Hit
TGTTGGTTGATTGTAGATATTGGGCT63900.1439179465003615No Hit
GATGTTCGCCGACCGTTGACTATTCT62230.14015671065285595No Hit
TGGGCTGTTAATTGTCAGTTCAGTGT58560.13189100073648152No Hit
CTACACCTCTCATGTCTCTTCACCGT54550.12285953022839935No Hit
TGAGGAGGGTGACGGGCGGTGTGTAC51880.11684605734645938No Hit
CACCTTGTTACGACTTTTACTTCCTC50260.11319743335067556No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)