FastQCFastQC Report
Wed 28 Aug 2024
HWTCTDMXY_n01_CITE-seq_test_BCells_300D28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTCTDMXY_n01_CITE-seq_test_BCells_300D28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483804
Sequences flagged as poor quality0
Sequence length26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACGACGCTCTTCCGATCTACACGA67181.38857884597895No Hit
GAGGTGATGTTTTTGGTAAACAGGCG10160.2100023976651702No Hit
GAGGTGATGCTAGAGGTGATGTTTTT10060.20793544493224528No Hit
GACAGAGGTTATCCGATCAGAGCAGT9330.19284668998189347No Hit
AAGACCTCAATAGCAATAACAGTTAA9080.18767930814958123No Hit
ACACCCCTTTGTCAACCAGCGCTGCT8230.17011020991971956No Hit
GCAAACTCATCGACGCCGCTAACTCA7570.1564683218824152No Hit
ATCCCCACACACCTGCTCAGGTGCGC7210.14902729204388554No Hit
GCTGCTGCTGCTGCTGCTGCTGCTGC7180.14840720622400805No Hit
ATGCGATGATTATGGTAGCGGAGGTG6980.14427330075815825No Hit
CATTCGCGACATGTCAAGTCCTGGTA6760.13972600474572347No Hit
CACACTCCACTCTTTCCCTACACGAC6650.13745235673950607Illumina Single End PCR Primer 1 (95% over 22bp)
GAATGAATCCTTTCTCCCCGACCGGG6580.13600548982645866No Hit
GACCAATGCGGTAGCCTCCCAGCCAA6350.13125149854073137No Hit
CTGCCTACTCGTCAACGCATCGCTCT6020.1244305545220792No Hit
CAGTCCTGAATCACAAAGTGGTAAGC5970.12339707815561675No Hit
GTGAAGGTCTTGTCATATCGCAATGA5960.12319038288232424No Hit
AAGACCTCAATAGCAATAGACCGGTC5930.12257029706244676No Hit
GCTACCTTTGCACGGTTAGGGTACCG5860.12112343014939934No Hit
TTGGCTCATCGGACCCGTAATGTTTT5820.12029664905622939No Hit
ATCCACCATTGTCCGAAGGACAATCC5440.11244222867111475No Hit
CTAACTTCACCATGTACACGAGGCCA5330.11016858066489736No Hit
TGCCCTACAGCCACCACTCCATACAG5280.10913510429843491No Hit
AACCGCGTCCTTTCTCCTCTACAGTG5270.10892840902514242No Hit
TACCTATCAACTTGACGGCAGCTCCT5260.10872171375184993No Hit
ACAGCCGCATACGCCGGGAGGGTTCA5210.10768823738538746No Hit
ACCATAAAAATTGTTGCCGCGGCTTC5210.10768823738538746No Hit
TGCGGGTTTTAGTTAAATGTCCTTTG5130.10603467519904755No Hit
TTTCCTCGCTGATTCTGCCAGGCCCG5070.1047945035592926No Hit
GACTAACGTACAGTTCTTCCCTTGCT5020.10376102719283015No Hit
CAGCAACAGCAGCAGCAGGAAACAAG4980.10293424609966019No Hit
GAACTCAGTCAAGCGCAGTAGCCGTT4930.10190076973319775No Hit
AGATTGCATCGAATTAAACCACATGC4880.10086729336673528No Hit
AGTCTTTTCTCTGTCGCCACAGTCAC4850.10024720754685781No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)