FastQCFastQC Report
Wed 28 Aug 2024
HWTCTDMXY_n01_CITE-seq_test_BCells_290D7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTCTDMXY_n01_CITE-seq_test_BCells_290D7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1355951
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGTTAATTGTCAGTTCAGTGTTT91690.676204376116836No Hit
GTGTTGGGTTGACAGTGAGGGTAATA72480.5345325900419705No Hit
ACACGACGCTCTTCCGATCTACACGA71320.525977708634014No Hit
ATCCACCTTCGACCCTTAAGTTTCAT63540.46860100401858185No Hit
AAACGGGGTTTATCGATTACAGAACA37680.2778861478032761No Hit
TGCTACCTTTGCACGGTTAGGGTACC35770.2638000930712098No Hit
GCTGCTGCTGCTGCTGCTGCTGCTGC31160.22980181437234826No Hit
AGGGGTTTTAGTTAAATGTCCTTTGA29050.21424078008718603No Hit
GACATCTTTCACCTCAAAGGTGATCA27070.19963848251153618No Hit
GGGCTGTTAATTGTCAGTTCAGTGTT24080.1775875381927518No Hit
AGTTGGTTGATTGTAGATATTGGGCT23360.1722776118016064No Hit
ACTTGTTGGTTGATTGTAGATATTGG23070.17013889144961727No Hit
GTGTTAGGTTGACAGTGAGGGTAATA22790.16807392007528296No Hit
AAACGGGGGAAGGCGCTTTGTGAAGT22750.16777892416466378No Hit
ATTGGGCTGTTAATTGTCAGTTCAGT21620.15944528968967167No Hit
GGGACCTAACGTCTTTACGTGGGTAC21580.1591502937790525No Hit
GACGCGGGCTTATGCGGAGGAGAATG21500.15856030195781412No Hit
TGGGGTTTTAGTTAAATGTCCTTTGA20040.14779295122021371No Hit
CCTCTATATAAATGCGTAGGGGTTTT19600.14454799620340264No Hit
TGGGCTGTTAATTGTCAGTTCAGTGT18140.13378064546580223No Hit
GGCTGGTTGATTGTAGATATTGGGCT17980.13260066182332547No Hit
GTTGGGTTGACAGTGAGGGTAATAAT17530.1292819578288596No Hit
AGTGGGTATAATGCTAAGTTGAGATG17280.12743823338748966No Hit
ATGAGGGTAATAATGACTTGTTGGTT17260.12729073543218006No Hit
CTACATTCACAGGCCTTCATTTTGGA16860.12434077632598817No Hit
AAACGGGGTTCGCTAATACAAGGTGG16090.1186621050465688No Hit
ACTTATACTAACATTAGTTCTTCTAT15510.11438466434259055No Hit
CGGCTGTTAATTGTCAGTTCAGTGTT15140.11165595216936305No Hit
TGGTTGATTGTAGATATTGGGCTGTT14820.10929598488440954No Hit
TTAGTTCTTCTATAGGGTGATAGATT14610.1077472563536588No Hit
AAGACCTAACGTCTTTACGTGGGTAC14430.10641977475587246No Hit
AGTGGGTGTTGAGCTTGAACGCTTTC14250.1050922931580861No Hit
GTGTTGGTTGATTGTAGATATTGGGC14160.10442855235919292No Hit
GGTGAAGTCTTAGCATGTACTGCTCG14080.10383856053795455No Hit
TAGTTGGTTGATTGTAGATATTGGGC13750.10140484427534624No Hit
CGGGCTGTTAATTGTCAGTTCAGTGT13580.10015111165521468No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)