FastQCFastQC Report
Wed 28 Aug 2024
HWTCTDMXY_n01_CITE-seq_test_BCells_253D28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTCTDMXY_n01_CITE-seq_test_BCells_253D28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866832
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTGGGTGTTGAGCTTGAACGCTT80500.9286689923768389No Hit
TTTGGTTGATTGTAGATATTGGGCTG75210.8676421728777894No Hit
ACACGACGCTCTTCCGATCTACACGA54190.6251499713900732No Hit
AGGGGTTTTAGTTAAATGTCCTTTGA28630.33028314598445835No Hit
AGAGTGGGTACTTGCGCTTACTTTGT18620.21480517562803403No Hit
AGTTGGTTGATTGTAGATATTGGGCT15520.1790427672259446No Hit
AGGGATTTTTTAGGTAGTGGGTGTTG15000.17304391162301344No Hit
AGAGGGATATGAAGCACCGCCAGGTC15000.17304391162301344No Hit
AGAGTGGGTATAATACTAAGTTGAGA14760.17027520903704524No Hit
CCTCTATATAAATGCGTAGGGGTTTT14590.16831404470531774No Hit
AGGGGTTTATCGATTACAGAACAGGC12940.14927921442678627No Hit
ACTTGTTGGTTGATTGTAGATATTGG10000.11536260774867564No Hit
CAGTCCTTTGAAGTATACTTGAGGAG9340.10774867563726305No Hit
GACGTAGGCTTATGCGGAGGAGAATG9250.10671041216752497No Hit
AGTGAGGGTAATAATGACTTGTTGGT9210.10624896173653026No Hit
TTTCCTCGTCCATGATCTGTAGGAAG8710.10048083134909648No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)