FastQCFastQC Report
Wed 28 Aug 2024
HWTCTDMXY_n01_CITE-seq_test_BCells_029D7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTCTDMXY_n01_CITE-seq_test_BCells_029D7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2141178
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATATAAATGCGTAGGGGTTTT85810.40076070275334413No Hit
AGCGGCTGCACCATCGGGATGTCCTG80670.3767552253946192No Hit
TGCTACCTTTGCACGGTTAGGGTACC62880.2936701199059583No Hit
TGGGGTTTTAGTTAAATGTCCTTTGA55940.2612580551453452No Hit
ATGAGGGTAATAATGACTTGTTGGTT53010.24757399898560511No Hit
ACGAGGAGTTCAGTTATATGTTTGGG52250.24402455097147457No Hit
GGGCTGTTAATTGTCAGTTCAGTGTT51690.2414091682242205No Hit
TACAGTGTTTTAATCTGACGCAGGCT46250.21600259296518085No Hit
GAGGTGATGTTTTTGGTAAACAGGCG44650.2085300708301692No Hit
AGGGGTTTTAGTTAAATGTCCTTTGA43620.20371963470575544No Hit
AGCGGTCCAATTGGGTGTGAGGAGTT42670.19928282468809227No Hit
TGGGCTGTTAATTGTCAGTTCAGTGT42050.19638722236077522No Hit
AGAGGTGATGTTTTTGGTAAACAGGC37780.17644492891296287No Hit
AGAGGGTAATAATGACTTGTTGGTTG37390.17462350164255377No Hit
TACAGTGAGGGTAATAATGACTTGTT29850.1394092410813113No Hit
GGGGTTTTAGTTAAATGTCCTTTGAA28860.13478561801027283No Hit
AGAGTGGGTGTTGAGCTTGAACGCTT27850.13006858841254676No Hit
ATCCACCTTCGACCCTTAAGTTTCAT25860.12077463900712598No Hit
ACGAGGAGGGTGACGGGCGGTGTGTA25680.11993398026693718No Hit
GGGGTAGAAAATGTAGCCCATTTCTT25420.11871969541999777No Hit
ACACGACGCTCTTCCGATCTACACGA25060.11703837793962016No Hit
TCTCTATATAAATGCGTAGGGGTTTT24290.11344222666214579No Hit
GTAGGCCTTATTTCTCTTGTCCTTTC24110.11260156792195698No Hit
ACGAGGAGAATGTTTTCATGTTACTT23900.11162079939173669No Hit
GACGTAGGCTTATGCGGAGGAGAATG23000.10741750569079264No Hit
CATGCCTGTGTTGGGTTGACAGTGAG22250.10391476094000593No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)