FastQCFastQC Report
Mon 20 Nov 2017
HWT7JBGX3_n02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWT7JBGX3_n02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388860798
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12659050.325541943675176No Hit
GTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTCCCCT6153150.15823528706537293No Hit
GTGTTATACCGGTGGCTTCAGGGGGTATTCATGTTTGGCATATGCCAGCT5682860.14614124203900852No Hit
TCCTTGGGGTAATGCACCTGGTGCAGCAGCTAATCGGGTGGCTTTAGAAG5679650.1460586932190578No Hit
GTCATATATAGCCTCGTTTAATTGTTCATCTCTGATTCGATGATGTCTCC5676450.14597640156053993No Hit
GAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTCCC5347780.13752427674645673No Hit
CCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGG4930880.12680321661017627No Hit
CCCCTAGTAACGGCGAGCGAACCGGGAGATGCCCAGCTTGAGAATCGGGC4625710.11895542116333363No Hit
CTTTAGGACATCCTTGGGGTAATGCACCTGGTGCAGCAGCTAATCGGGTG4296630.11049275273050281No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG2216250.019.8561765
ATACCGG2137250.019.5108156
GTTATAC2247700.019.3327663
TTATACC2283500.018.7792974
GGTATCA754750.018.3642331
TACCGGT2267100.018.235847
GTCATAT2608050.017.9231621
GGACCAT1013950.017.2078766
TGTTATA2732750.017.1303162
TAGTCCA832450.017.0000699
GGTATTC2271850.016.85990524
TCAGGGG2412900.016.80939518
TTAGTCC811350.016.728738
GTAGATC2051200.016.67765443
GGCTTCA2443300.016.51958714
GGGGGTA2434800.016.47820721
CCGGTGG2455900.016.4243899
ACCGGTG2598250.016.294678
TTCAGGG2490700.016.19500217
TGGCTTC2475600.016.12705213
Produced by FastQC (version 0.11.2)