FastQCFastQC Report
Mon 20 Nov 2017
HWT7JBGX3_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWT7JBGX3_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388860798
Sequences flagged as poor quality0
Sequence length20
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCTT9794150.013.9409521
CCTACAA6249650.013.9223241
TTCCAGA7568600.013.8612371
ACGGAAT9250700.013.8553322
CCTACGT5396750.013.8525226
CCTCTCT3071150.013.8469324
TTCGACC4983950.013.8451241
CTCCTAC5418050.013.8381834
GTCCTCT4681400.013.8364472
AATCCTT4448750.013.8317462
GGCATCT5309050.013.8312226
CCTCTGA4678500.013.8308084
CACCTTC4060400.013.8271843
TCGACCA5005850.013.8261692
GAGGAAC8166050.013.8256221
TTCCACC2887850.013.8238482
CTTCTTG9869900.013.8203152
ACACCTT4069600.013.8202282
AGGAACA8188700.013.8179892
TCCTACG5416650.013.8175195
Produced by FastQC (version 0.11.2)