FastQCFastQC Report
Tue 30 Aug 2016
HWT5WBCXX_l02n02_mesp_mekmut_10hpf_3_june_2016.3520000006799b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWT5WBCXX_l02n02_mesp_mekmut_10hpf_3_june_2016.3520000006799b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61394745
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGTC4066870.6624133710466589Illumina Single End PCR Primer 1 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCTC536750.036.52033238
TCTCGGT539100.036.27841
GTAGATC550650.035.77051536
TCGGTGG549950.035.5069143
CTCGGTG555200.035.1225342
CTGCCGA564750.034.6849824
ATCTCGG574300.034.05444740
CCGACGA567050.033.85412627
GACGAGT572700.033.62183829
CGCTGCC587600.033.37984522
GCTGCCG590600.033.2891823
CGGTGGT596950.032.84215544
ACGAGTG587650.032.7697830
GGTGGTC597100.032.713845
CGAGTGT589400.032.7108931
GATCTCG601300.032.60334839
ACGCTGC615050.032.1532421
GTGTAGA618750.031.86270334
CTGACGC640150.031.04919618
GAGTGTA641250.030.92940532
Produced by FastQC (version 0.11.2)