FastQCFastQC Report
Tue 30 Aug 2016
HWT5WBCXX_l02n02_gfp_10hpf_2_june.352000000679de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWT5WBCXX_l02n02_gfp_10hpf_2_june.352000000679de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3412
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATGGTTTCCGTTGCTGCCATCTCAAAAACATTTGGACTGCTCCGCTTCC40.11723329425556857No Hit
CAGACTTTTATTTCTCGCCATAATTCAAACTTTTTTTCTGATAAGCTGGTT40.11723329425556857No Hit
TAAAGGTAAAAAACGTTCTGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCG40.11723329425556857No Hit
TATTTCTCGCCATAATTCAAACTTTTTTTCTGATAAGCTGGTTCTCACTTC40.11723329425556857No Hit
CGCTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAACACTGACGT40.11723329425556857No Hit
TGGATGGTCGCCATGATGGTGGTTATTATACCGTCAAGGACTGTGTGACTA40.11723329425556857No Hit
GTGGATGCCTGACCGTACCGAGGCTAACCCTAATGAGCTTAATCAAGATGA40.11723329425556857No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)