FastQCFastQC Report
Tue 30 Aug 2016
HWT5WBCXX_l02n02_3xbfp_5hpf_26_july_2016.35200000067964.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWT5WBCXX_l02n02_3xbfp_5hpf_26_july_2016.35200000067964.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9749406
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGTC237400.24350201437913244Illumina Single End PCR Primer 1 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCCGA39650.030.07188224
TCGGTGG40000.029.58226643
CCGACGA40000.028.85247627
TCTCGGT41200.028.82807441
GTAGATC41750.028.50671836
ATCTCGG41650.028.46259740
GACGAGT41100.028.0799829
CTCGGTG43400.027.31645442
ACGAGTG42600.026.93254730
AGATCTC44550.026.76472538
TGCCGAC43350.026.72674425
CGCTGCC45800.026.32640822
CTGACGC46300.026.23779518
GCTGCCG47250.025.71158423
GCCGACG45400.025.52005226
GTGTAGA47200.025.31048634
CGGTGGT47850.025.19839744
TGACGCT48350.025.17173419
CGAGTGT46200.025.1258131
GAGTGTA48250.025.08454332
Produced by FastQC (version 0.11.2)