FastQCFastQC Report
Tue 30 Aug 2016
HWT5WBCXX_l02n02_3xbfp_5hpf_21_july_2016.3520000006794b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWT5WBCXX_l02n02_3xbfp_5hpf_21_july_2016.3520000006794b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6283540
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGTC359710.5724639295683643Illumina Single End PCR Primer 1 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG45450.037.91456643
CGGTGGT48500.035.62159744
CTCGGTG49050.035.1777142
TCTCGGT49800.034.69088741
GGTGGTC50800.034.05363545
CCGACGA50300.033.7234427
CTGCCGA51650.033.4953524
ACGAGTG51350.033.1639830
GCTGCCG52600.033.01896723
GACGAGT51700.032.98350529
CGAGTGT52950.032.4595231
ATCTCGG53250.032.40106640
CGCTGCC53850.032.24891722
TGCCGAC53350.031.92198825
ACGCTGC54850.031.86501121
GTGTAGA55200.031.62801234
AGATCTC55250.031.43575538
GCCGACG53950.031.27507226
GTAGATC56700.030.63257436
CGACGAG56350.030.4620528
Produced by FastQC (version 0.11.2)