FastQCFastQC Report
Tue 30 Aug 2016
HWT5WBCXX_l02n02_3xbfp_5hpf_16_aug_2016.35200000067971.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWT5WBCXX_l02n02_3xbfp_5hpf_16_aug_2016.35200000067971.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5858875
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG10450.015.504037
CTGACGC14550.015.30684545
AGTCTGA11850.014.99889633
GCGGTAG9700.014.14856411
GGCACGT10400.013.41144420
CGGTAGC10450.013.13311712
GGACGAC12550.013.0890327
CTCACTC11700.013.0767418
ACCGCGG10700.013.0372038
TATACTG18000.012.88637355
CTAGTCA13800.012.87959612
TAGACTC6000.012.7612645
CCGCGGT10800.012.7078319
GTACTAC15300.012.6483966
GTCTGAA14450.012.30013334
CCTACTC8700.012.1663273
GTATAAG24650.012.1509951
TCTGACG21250.011.96280345
TAGCTGG11300.011.94551315
CGATTAG8750.011.82729714
Produced by FastQC (version 0.11.2)