FastQCFastQC Report
Tue 30 Aug 2016
HWT5WBCXX_l02n01_mesp_mekmut_10hpf_3_june_2016.3510000006799e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWT5WBCXX_l02n01_mesp_mekmut_10hpf_3_june_2016.3510000006799e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61394745
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCGTGATATCTCGTAT4008270.6528685802017746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACG543300.036.59980429
AGACGTC545100.036.35696431
GAGACGT547600.036.28795230
TCCGAGC555750.036.0375119
TATCTCG530250.035.9303142
CCACGAG561000.035.49043326
GCCCACG561700.035.4381924
CCCACGA563050.035.26526625
CGAGCCC565200.035.2626121
CCGAGCC568050.035.16214420
AGCCCAC567750.035.1075923
CTCCGAG577450.034.55694618
ACGAGAC580850.034.31783328
CACGAGA585450.034.0697727
GAGCCCA591250.033.79868722
ACGTCGT586600.033.79074533
ATATCTC571850.033.3912641
CTCGTAT590900.033.247545
CGTGATA596000.033.19618237
CGTCGTG604150.032.91153334
Produced by FastQC (version 0.11.2)