FastQCFastQC Report
Tue 30 Aug 2016
HWT5WBCXX_l02n01_3xbfp_5hpf_26_july_2016.35100000067967.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWT5WBCXX_l02n01_3xbfp_5hpf_26_july_2016.35100000067967.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9749406
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTAT233430.23942997142595146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCTA36300.032.5433932
TACGCTA38450.030.77398137
CTCCGAG39650.030.014218
CTACGCT39550.029.97540736
TCCGAGC40100.029.78805719
GCTACGC39800.029.7327335
AGCCCAC40650.029.46075623
CGCTACG40950.028.843434
ACGCTAC41700.028.54483233
TATCTCG40250.028.28040142
CCGAGCC43050.027.79908620
CCACGAG43600.027.20983126
CGCTATC42300.027.01620139
CACGAGA43950.026.94192527
GCCCACG44350.026.6481724
CGAGACG45050.026.53283329
GAGCCCA45750.026.51736322
CTATCTC43050.026.38877341
CCCACGA44950.026.3425525
ACGCTAT44250.025.926838
Produced by FastQC (version 0.11.2)