FastQCFastQC Report
Tue 30 Aug 2016
HWT5WBCXX_l02n01_3xbfp_5hpf_21_july_2016.3510000006794e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWT5WBCXX_l02n01_3xbfp_5hpf_21_july_2016.3510000006794e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6283540
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTAT339440.5402050436537366No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGGC60650.028.75317633
GAGACTA61950.028.44853230
CTAGGCA62550.028.19883234
CGAGACT62600.027.97670229
TAGGCAT63450.027.79818335
GCCCACG63800.027.7687124
TGATCTC59250.027.64081441
CCCACGA64400.027.57990625
CCACGAG64300.027.55277826
AGCCCAC64650.027.47281823
CCGAGCC65400.027.17292620
CACGAGA64850.027.04139727
AGACTAG65050.026.91314931
CGAGCCC66150.026.8774621
CATGATC61600.026.80545239
ATGATCT62000.026.7050840
AGGCATG65900.026.69388836
GGCATGA66250.026.55265237
TCCGAGC67150.026.53176919
CTCGTAT64900.026.5170145
Produced by FastQC (version 0.11.2)