FastQCFastQC Report
Tue 30 Aug 2016
HWT5WBCXX_l02n01_3xbfp_5hpf_16_aug_2016.35100000067974.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWT5WBCXX_l02n01_3xbfp_5hpf_16_aug_2016.35100000067974.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5858875
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAG10900.020.22633745
AACCGCG9300.015.2400517
ACCGCGG9750.015.2283668
AGTCTGA12350.014.5754633
TATACCG12500.013.6788925
GCGGTAG9800.013.31422611
CCTACTC9850.013.0198063
TAGACCG10250.012.95017055
TAGATAG7900.012.8154185
CGTCCAG12200.012.72340210
GGCACGT10700.012.40405620
CCTATTC16700.012.1238359
GTCGTCC11350.012.0906038
TCGTCCA13650.011.701459
GGACGAC13100.011.6779457
TGTAGAG16000.011.67043217
GTACTAC14750.011.5917866
CCGCGGT11100.011.5522329
TCTCCGA22250.011.5263745
TTAGTCG11550.011.4925765
Produced by FastQC (version 0.11.2)