FastQCFastQC Report
Tue 30 Aug 2016
HWT5WBCXX_l01n02_mesp_mekmut_10hpf_3_may_2016_1.352000000679a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWT5WBCXX_l01n02_mesp_mekmut_10hpf_3_may_2016_1.352000000679a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3850180
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGTC67040.17412172937369164Illumina Single End PCR Primer 1 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGT10250.030.50503541
CTGCCGA11200.027.51657924
ACGAGTG11350.026.95472530
CGAGTGT11400.026.63848531
GACGAGT11650.026.26027129
CTCGGTG11800.026.1167542
GCTGCCG11750.026.0371223
TCGGTGG12250.025.34099243
CGGTGGT12250.025.34000444
ACGCTGC12300.025.23961621
GTAGATC12250.025.15834236
CGCTGCC12500.024.47520822
GCCGACG13550.022.90975426
CCGACGA13250.022.74937627
CGACGAG14000.022.49501228
TGCCGAC13650.022.24810225
AGATCTC14400.021.714538
GGTGGTC14750.021.04590645
GTGTAGA14700.020.96528434
GAGTGTA15300.020.58420632
Produced by FastQC (version 0.11.2)