FastQCFastQC Report
Tue 30 Aug 2016
HWT5WBCXX_l01n02_3xbfp_5hpf_26_july_2016.35200000067958.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWT5WBCXX_l01n02_3xbfp_5hpf_26_july_2016.35200000067958.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10932378
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGTC317670.29057721933873853Illumina Single End PCR Primer 1 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGG51900.030.85958940
CCGACGA51100.030.77058627
TCGGTGG52250.030.7391243
TCTCGGT52350.030.59446141
GTAGATC52650.030.37824436
CTGCCGA52450.030.36541424
GACGAGT52800.029.90795129
CTCGGTG54100.029.52164842
ACGAGTG55550.028.63009630
AGATCTC56950.028.36065138
CGCTGCC57900.027.8953222
CGAGTGT57400.027.66790631
GAGTGTA60150.026.88986632
GCTGCCG61100.026.8759123
CTGACGC59950.026.87007718
GTGTAGA59950.026.86675834
ACGCTGC61000.026.55241421
TGCCGAC59700.026.33869625
TGACGCT61200.026.31969319
GCCGACG59550.026.29123326
Produced by FastQC (version 0.11.2)