FastQCFastQC Report
Tue 30 Aug 2016
HWT5WBCXX_l01n02_3xbfp_5hpf_21_july_2016.3520000006793e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWT5WBCXX_l01n02_3xbfp_5hpf_21_july_2016.3520000006793e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7569754
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGTC514840.6801277822238345Illumina Single End PCR Primer 1 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG64800.037.97780643
CGGTGGT67400.036.5444744
TCTCGGT68900.035.78341341
GGTGGTC69950.035.24558345
CTCGGTG70050.035.1638542
ATCTCGG72800.033.92802440
CTGCCGA73000.033.46572524
CCGACGA72650.033.16183527
GACGAGT73150.032.935629
CGCTGCC75050.032.85199722
AGATCTC75350.032.7802638
GCTGCCG76200.032.29651623
ACGAGTG75300.032.0848330
CGAGTGT76100.031.8064531
TGCCGAC76450.031.69109525
GCCGACG76100.031.6288826
GATCTCG78450.031.57053639
ACGCTGC78700.031.50070421
GTGTAGA78750.031.45068234
GTAGATC79150.031.26311136
Produced by FastQC (version 0.11.2)