FastQCFastQC Report
Tue 30 Aug 2016
HWT5WBCXX_l01n02_3xbfp_5hpf_16_aug_2016.3520000006798e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWT5WBCXX_l01n02_3xbfp_5hpf_16_aug_2016.3520000006798e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6499699
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACGC17950.015.41347945
AACCGCG11850.014.6269327
TCTGACG24700.013.0226445
TCACGTC13700.012.4926494
GCGGTAG11900.012.48046411
ACCGCGG12500.012.4246238
TATACTG19250.012.40004355
TAGACGG4850.012.0720155
TACCTGG14400.011.884912
TATGGTC11400.011.83803644
CGACTAA15800.011.821197510
CCTATTC15450.011.6532959
GGCACGT12750.011.64547520
GTATAAG27150.011.6116231
GTCGTCC11650.011.5922958
TAGACCG9950.011.54237655
CCTACTC9800.011.489263
TATACCG15100.011.4831725
CTAAGGC12200.011.4440563
AGTCTGA12450.011.2025233
Produced by FastQC (version 0.11.2)