FastQCFastQC Report
Tue 30 Aug 2016
HWT5WBCXX_l01n01_mesp_mekmut_10hpf_3_may_2016_1.351000000679ab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWT5WBCXX_l01n01_mesp_mekmut_10hpf_3_may_2016_1.351000000679ab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3850180
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTAT67440.17516064184012178No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACT9100.033.37429434
CGTACTA9300.032.17276835
GACCGTA9800.031.45647832
ACCGTAC10100.030.74491733
CCCACGA10900.028.70640625
GAGACCG10900.028.49544130
CCACGAG11150.027.86087426
ACGAGAC11600.027.36224228
GTACTAG11050.027.07718336
CGAGCCC11450.026.92519621
GCCCACG11800.026.51693324
CCGAGCC11900.026.08730920
CTAGATC11450.025.34469239
TCTCGTA11950.024.84867344
CACGAGA13000.024.76185827
CTCGTAT12500.024.47519145
AGCCCAC13450.023.59804723
TCCGAGC13650.023.07238819
CGAGACC14700.021.59197229
AGACCGT14700.021.58383831
Produced by FastQC (version 0.11.2)