FastQCFastQC Report
Tue 30 Aug 2016
HWT5WBCXX_l01n01_3xbfp_5hpf_26_july_2016.3510000006795b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWT5WBCXX_l01n01_3xbfp_5hpf_26_july_2016.3510000006795b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10932378
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTAT322900.2953611739367226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCTA50750.031.25955232
TACGCTA51050.031.11110737
GCTACGC51600.030.82619535
TCCGAGC53250.030.62788219
CGCTACG51950.030.61851134
TATCTCG51500.030.22716542
CTACGCT52400.030.22468636
CTCCGAG53950.029.94038218
AGCCCAC54100.029.7488123
CCGAGCC55500.029.18381320
ACGCTAC54900.029.18349633
CACGAGA55400.028.84824227
CTATCTC54400.028.69849441
CGAGACG56150.028.66335729
CCACGAG56600.028.27638826
AGACGCT56900.028.0793931
GCCCACG57800.027.80616824
ACGCTAT56350.027.70576138
GCTATCT56400.027.60117140
CGCTATC56600.027.50389339
Produced by FastQC (version 0.11.2)