FastQCFastQC Report
Tue 30 Aug 2016
HWT5WBCXX_l01n01_3xbfp_5hpf_21_july_2016.35100000067931.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWT5WBCXX_l01n01_3xbfp_5hpf_21_july_2016.35100000067931.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7569754
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTAT502980.6644601660767312No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGGC86000.028.33718533
CGAGACT87350.027.98798229
GAGACTA88100.027.6697730
CTAGGCA89000.027.65463434
TGATCTC86900.027.59438341
AGCCCAC91400.027.33802223
CCCACGA91250.027.30985625
TAGGCAT90100.027.2670735
GCCCACG91250.027.26033424
CCACGAG91600.027.107226
CTCGTAT90950.027.0086745
CCGAGCC92950.026.93574720
TCTCGTA89950.026.78375844
ACGAGAC92500.026.62442628
AGACTAG91500.026.61102331
CATGATC89850.026.58824739
CACGAGA92150.026.50568627
GGCATGA93050.026.44834737
AGGCATG93250.026.3931936
CGAGCCC94700.026.35550721
Produced by FastQC (version 0.11.2)