FastQCFastQC Report
Tue 30 Aug 2016
HWT5WBCXX_l01n01_3xbfp_5hpf_16_aug_2016.35100000067981.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWT5WBCXX_l01n01_3xbfp_5hpf_16_aug_2016.35100000067981.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6499699
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAG12050.023.14960545
CTAGTCA15850.015.18810512
GTACTAC17950.014.915176
ACTAGTC17350.014.52292811
AGTCTGA14050.014.41280933
AACCGCG11400.014.2096497
TATACCG14550.014.0708645
CCGTGCG6550.013.73897659
CCTATTC16500.013.3621959
CTACTAG18850.013.1285569
TACCTGG13600.013.0686452
GTCTTAG9850.012.7923681
GCGGTAG11100.012.76889211
CCGTCCG3601.8189894E-1212.49865159
AGTCCTC6000.012.37395135
GGCACGT11150.012.307425520
TAGACTC5850.012.3065545
GTCTGAA16100.012.29709434
ATAGTAC26200.012.1935293
GTATAAG28900.012.0679361
Produced by FastQC (version 0.11.2)