FastQCFastQC Report
Tue 21 Dec 2021
HWT3FBGXK_n01_DO11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWT3FBGXK_n01_DO11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4525333
Sequences flagged as poor quality0
Sequence length76
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2460355.436837465883726No Hit
TGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1458953.2239616399500326No Hit
TGTGTTGGGTGTGTTTGGTGTGTTGGGTGTGTTGGGTGTGTTGGGTGTGT659561.4574839022896215No Hit
TGTGTTGGGTGTGTTTGGTGTGTTGGGTGTGTTTGGTGTGTTGGGTGTGT576461.2738510072076465No Hit
TGTGTTGGGTGTGTTTGGTGTGTTGGGTGTGTTGGGTGTGTTTGGTGTGT426630.9427593505273535No Hit
TGTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG403950.8926414917973993No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTTGG302610.6687021706468894TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGTGG270090.5968400557483835TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC191680.42357103886056563TruSeq Adapter, Index 10 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTTTG170910.3776738640007266TruSeq Adapter, Index 10 (97% over 42bp)
TGTGTTGGGTGTGTTTGGGTGTGTTGGGTGTGTTGGGTGTGTTGGGTGTG163790.36194021522836No Hit
TGTGTTGGGTGTGTTGGGTGTGTTGGGTGTGTTGGGTGTGTTGGGTGTGT146650.32406454950387076No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGATGG128030.28291840622557496TruSeq Adapter, Index 10 (97% over 42bp)
TGTGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG124520.27516207094593925No Hit
TGTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG117550.25975988949321516No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGC117280.2591632483178586TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTTTGG116550.25755010736226486TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGTTG109820.2426782736209689TruSeq Adapter, Index 10 (97% over 42bp)
TGTGTTGGGTGTGTTTGGTGTGTTGGGTGTGTTGGTGTGTTGGGTGTGTT107140.2367560575100219No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGTTGG105970.23417061241681TruSeq Adapter, Index 10 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGG105950.234126416774191TruSeq Adapter, Index 10 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGATGG104820.23162936296621706TruSeq Adapter, Index 10 (97% over 45bp)
TGTGTTGGGTGTGTTTGGTGTGTTGGGTGTGTTTGGTGTGTTTGGTGTGT102230.22590602724705564No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGG97730.21596200765777898TruSeq Adapter, Index 10 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTTTTG82940.18327932994102314TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGATGC78630.17375516895662704TruSeq Adapter, Index 10 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTTTGG78610.17371097331400803TruSeq Adapter, Index 10 (97% over 46bp)
TGTGTTGGGTGTGTTTGGTGTGTTGGGTGTGTTGGGTGTGTTGGTGTGTT78520.17351209292222253No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTATGG77240.17068357179460605TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGATTG71970.15903801996449765TruSeq Adapter, Index 10 (97% over 42bp)
TGTGTTGGGTGTGTTTGGTGTGTTGGGTGTGTTTGATGTGTTGGGTGTGT71930.15894962867925963No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATTC67120.14832057662938838TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGGTGG62970.13914998078594437TruSeq Adapter, Index 10 (97% over 45bp)
TGTGTTGGGTGTGTTTGGTGTGTTGGGTGTGTTGGGTGTGTTTGGGTGTG61680.1362993618370184No Hit
TGTGTTGGGTGTGTTTGGTGTGTTTGGTGTGTTGGGTGTGTTTGGTGTGT60950.13468622088142465No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGTTTG60510.13371391674380648TruSeq Adapter, Index 10 (97% over 45bp)
TGTGTTGGGTGTGTTTGGTGTGTTGGTGTGTTGGGTGTGTTGGGTGTGTT60070.13274161260618833No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTGTGG57440.12692988560178886TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATTG55390.12239983223334062TruSeq Adapter, Index 10 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATTG55110.12178109323667452TruSeq Adapter, Index 10 (97% over 48bp)
TGTGTTGGGTGTGTTTGGTGTGTTGGGTGTGTTGGGTGTGTTTGGTGTTT54830.1211623542400084No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTTGC54570.12058781088596132TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGATGC53340.11786977886489237TruSeq Adapter, Index 10 (97% over 42bp)
TGTGTTGGGTGTGTTTGGTGTGTTGGGTGTGTTGGGTGTGTGTTGGGTGT53160.11747201808132131No Hit
TGTGTTGGGTGTGTTTGGGTGTGTTTGGTGTGTTGGGTGTGTTTGGTGTG52890.11687537690596471No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGATTG52340.115659996733942TruSeq Adapter, Index 10 (97% over 45bp)
TGTGTTGGGTGTGTTTGGTGTGTTGGTGTGTTGGGTGTGTTTGGTGTGTT50800.11225693225227844No Hit
TGTGTTGGGTGTGTTTGGGTGTGTTGGGTGTGTTGGGTGTGTTTGGTGTG50300.11115204118680327No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTTTTG49650.10971568280168553TruSeq Adapter, Index 10 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTATTG49110.10852240045097233TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTTTGG48290.10671037910359303TruSeq Adapter, Index 10 (97% over 49bp)
TGTGTTGGGTGTGTTTGGTGTGTTGGGTGTGTTTGGTGTGTTTGGTGTTT47150.10419122747430962No Hit
TGTGTTGGGTGTGTTTGGTGTGTTGGGTGTGTTTGGTGTGTTTGGGGTGT46820.103461999371096No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTTTGC46460.10266647780395387TruSeq Adapter, Index 10 (97% over 46bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)