FastQCFastQC Report
Tue 21 Dec 2021
HWT3FBGXK_n01_DO10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWT3FBGXK_n01_DO10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2801309
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC673882.405589672542372TruSeq Adapter, Index 9 (100% over 50bp)
TGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG242310.8649884750307802No Hit
TGTGTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82530.29461226876435265No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)