FastQCFastQC Report
Mon 11 Sep 2023
HWNHNBGXT_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNHNBGXT_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88594719
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGATATCGTTCAGCGGTTAGGATATGTGAGATCGGAAGAGCACACGTCT2334120.2634603988077438Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCAGATCGGAAGAGCACACGTCT1811660.204488486497711Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGATATCGTTCAGCGGTTAGGATATGTAGATCGGAAGAGCACACGTCTG1464800.1653371686860929Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGATATCGTTCAGCGGTTAGGATATGAGATCGGAAGAGCACACGTCTGA1441550.1627128587653176Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)