FastQCFastQC Report
Mon 11 Sep 2023
HWNHNBGXT_n01_67192_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNHNBGXT_n01_67192_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54701702
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGATATCGTTCAGCGGTTAGGATATGAGATCGGAAGAGCACACGTCTGA18404493.364518712781551Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGAGATCGGAAGAGCACACGTCT15567662.845918761357736Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGATATCGTTCAGCGGTTAGGATATGTAGATCGGAAGAGCACACGTCTG13241012.420584646525258Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCAGATCGGAAGAGCACACGTCT11480652.098773818774414Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGGC9769291.785920664772003No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTGGAGATCGGAAGAGCACACGTC4621990.844944458949376No Hit
CGGACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATC4309700.7878548276249246TruSeq Adapter, Index 9 (100% over 42bp)
GCAAGCTTACGATGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3948470.7218184911321406Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TACTTGGTTGATCCTGCCAAGGAAGCACACTTAGATCGGAAGAGCACACGT3850430.7038958312485414No Hit
CGGGGAGAGTCGGATACTTTGAGAGTAGATCGGAAGAGCACACGTCTGAAC3275050.5987108042817388Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGTCTCTAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA3260590.5960673764776094TruSeq Adapter, Index 9 (100% over 38bp)
TCCGATATCGTTCAGCGGTTAGGATATGCAGATCGGAAGAGCACACGTCTG3043220.5563300388715511Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAAACTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAT2853280.5216071704679317TruSeq Adapter, Index 9 (100% over 41bp)
TACATCTTTTTTAATCGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGT2727710.4986517604150599Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGTTGGACCGATACGAATAATCATACTCAAAAGGTAGATCGGAAGAGCACA2499670.4569638436478631No Hit
TTTTTATGACTGACAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2449710.4478306726178282Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCACCGCTGGTCTAATGGTAGAATTTCACTAGATCGGAAGAGCACACGTCT2214940.40491244678273447Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTAGATCGGAAGAGCACACGTCTGAACT2073320.37902294155308003Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGGGGAGAGTCGGATACTTTGAGAGTAGATCGGAAGAGCACACGTCTGAA2021720.36958996266697514Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCGATGAGGATTACCCCGACGGGCGGTCTGAAATATGACGATAGTCGGT1907940.3487898786037773No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC1869310.3417279411159821TruSeq Adapter, Index 9 (100% over 50bp)
TGGACCGATACGAATAATCATACTCAAAAGGTAGATCGGAAGAGCACACGT1756090.32103023046705204No Hit
AGTGATTGTAAGATCTCTTACGTCAAGCTCTTCGATTTATAATCCTGAAAT1696250.31009089991386374No Hit
GCCTGTGTAGCTCAACCGGATAGAGCATAAGATCGGAAGAGCACACGTCTG1688620.3086960621444649Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGACTTTAGCTCACCTGGGAGAGCGTGCGAAGATCGGAAGAGCACACGTC1679860.3070946494498471No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTAAGATCGGAAGAGCACACGTCT1608150.293985368133518Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGATATCGTTCAGCGGTTAGGATAAGATCGGAAGAGCACACGTCTGAAC1393560.25475624140543196Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGATATCGTTCAGCGGTTAGGATATAAGATCGGAAGAGCACACGTCTGA1391250.25433395107157725Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGGAATGCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA1372340.2508770202433555TruSeq Adapter, Index 9 (100% over 38bp)
GGGACTTTAGCTCACCTGGGAGAGCGTGCAGATCGGAAGAGCACACGTCTG1349280.24666142929154194Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACCGCTGGTCTAATGGTAGAATTTCAGATCGGAAGAGCACACGTCTGAA1330660.24325751326713746Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGACTCTTAATCTAGATCGGAA1276450.23334740114667732No Hit
CTTCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAT1251130.22871866034442587TruSeq Adapter, Index 9 (100% over 41bp)
CGGGGAGAGTCGGATACTTTGAGAGTAGTGTCTTAAGGGGGTGGTATGTCC1077270.19693537140763917No Hit
GATGTTGGACCGATACGAATAATCATACTCAAAAGGTAGATCGGAAGAGCA1020710.18659565656659094No Hit
GCACCGCTGGTCTAATGGTAGAATTTCACAAGATCGGAAGAGCACACGTCT995860.1820528363084571Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATC969060.1771535371970693TruSeq Adapter, Index 9 (100% over 42bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGAGATCGGAAGAGCACACGTC956400.17483916679594358No Hit
CGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCT953780.1743602054649049TruSeq Adapter, Index 9 (100% over 43bp)
TTTTTTATGACTGACAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC937490.1713822359677218Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTC922680.16867482477967505TruSeq Adapter, Index 9 (100% over 44bp)
GGGACTTTAGCTCACCTGGGAGAGCGTGAGATCGGAAGAGCACACGTCTGA919400.16807520906753504Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTCGATTCTGGCCTGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT908290.16604419365232914Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGAAGATCGGAAGAGCACACGT891410.16295836645082817No Hit
GGGACTTTAGCTCACCTGGGAGAGCGTGCGAGATCGGAAGAGCACACGTCT872400.15948315465577287Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCAAGATCGGAAGAGCACACGTCTGA866820.15846307670646154Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGAGAGTCGGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG858240.1568945697521441Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATGTTGGACCGATACGAATAATCATACTCAAAAGGTAGATCGGAAGAGCAC836890.15299158333318402No Hit
CGGGGAGAGTCGGATACTTTGAGAAGATCGGAAGAGCACACGTCTGAACTC778090.14224237483506455Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TACTTGGTTGATCCTGCCAAGGAAGCACACTCAGATCGGAAGAGCACACGT764080.13968121138168607No Hit
CGGGGAGAGTCGGATACTTTGAGAGCAGATCGGAAGAGCACACGTCTGAAC727420.13297940894051158Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAAACTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAT691110.12634158988325445TruSeq Adapter, Index 9 (100% over 41bp)
CGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTC640480.1170859363754349TruSeq Adapter, Index 9 (100% over 44bp)
TTCGGGTCTTTCAGGATATTGTTAGATCGGAAGAGCACACGTCTGAACTCC621250.11357050645334583Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTTATGACTGACAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC601070.10988140734633815Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGACCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATC591190.10807524782318473TruSeq Adapter, Index 9 (100% over 42bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGAAGATCGGAAGAGCACACGTC582930.10656523996273462No Hit
GCACCGCTGGTCTAATGGTAGAATTTAGATCGGAAGAGCACACGTCTGAAC580330.10608993482506265Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGGCGTAGAGAAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG570960.10437700823276029Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCACCGCTGGTCTAATGGTAGAATTTCACAGATCGGAAGAGCACACGTCTG557410.10189993722681608Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTC557250.10187068767988244TruSeq Adapter, Index 9 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)