FastQCFastQC Report
Mon 11 Sep 2023
HWNHNBGXT_n01_67192_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNHNBGXT_n01_67192_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70044314
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGATATCGTTCAGCGGTTAGGATATGAGATCGGAAGAGCACACGTCTGA20157102.877763925277361Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGAGATCGGAAGAGCACACGTCT19403012.770104936711922Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCAGATCGGAAGAGCACACGTCT17355502.4777885611100423Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGATATCGTTCAGCGGTTAGGATATGTAGATCGGAAGAGCACACGTCTG16560052.3642247392129505Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATC9698221.3845834795383962TruSeq Adapter, Index 8 (100% over 42bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGGAGATCGGAAGAGCACACGTC6899010.9849493279354552No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGGC6246960.8918582599010106No Hit
GCAAGCTTACGATGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5577480.7962787671815874Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TACTTGGTTGATCCTGCCAAGGAAGCACACTTAGATCGGAAGAGCACACGT4014420.5731257500787288No Hit
GGAAACTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAT3698850.5280728425722037TruSeq Adapter, Index 8 (100% over 41bp)
TCCGATATCGTTCAGCGGTTAGGATATGCAGATCGGAAGAGCACACGTCTG3635650.5190499831292517Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTTGGACCGATACGAATAATCATACTCAAAAGGTAGATCGGAAGAGCACA3378360.4823175225900564No Hit
TACATCTTTTTTAATCGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGT3271850.467111434626942Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGGAGAGTCGGATACTTTGAGAGTAGATCGGAAGAGCACACGTCTGAAC3123680.4459576833031729Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACCGCTGGTCTAATGGTAGAATTTCACTAGATCGGAAGAGCACACGTCT3044020.43458488293567976Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGTCTCTAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG2964020.42316354186865185TruSeq Adapter, Index 8 (100% over 38bp)
TTTTTATGACTGACAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2959860.42256963213316645Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTCGATGAGGATTACCCCGACGGGCGGTCTGAAATATGACGATAGTCGGT2440770.34846083295212227No Hit
GCACCGCTGGTCTAATGGTAGAATTAGATCGGAAGAGCACACGTCTGAACT2422120.3457982328158714Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGACCGATACGAATAATCATACTCAAAAGGTAGATCGGAAGAGCACACGT2251460.32143365698463405No Hit
GGGACTTTAGCTCACCTGGGAGAGCGTGCGAAGATCGGAAGAGCACACGTC2216830.3164896439702443No Hit
CGGACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATC2090890.2985095977954756TruSeq Adapter, Index 8 (100% over 42bp)
AGTGATTGTAAGATCTCTTACGTCAAGCTCTTCGATTTATAATCCTGAAAT1974950.28195721925408535No Hit
CTTCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAT1938730.27678620708598844TruSeq Adapter, Index 8 (100% over 41bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGATCGGAAGAGCACACGTCTG1923990.2746818249943886Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGAATGCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG1897240.2708628140751011TruSeq Adapter, Index 8 (100% over 38bp)
TCCGATATCGTTCAGCGGTTAGGATATGTAAGATCGGAAGAGCACACGTCT1790080.25556392771581715Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGATATCGTTCAGCGGTTAGGATAAGATCGGAAGAGCACACGTCTGAAC1592690.22738319630055912Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGACTTTAGCTCACCTGGGAGAGCGTGCAGATCGGAAGAGCACACGTCTG1566490.22364270710110745Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC1557300.2223306805460326TruSeq Adapter, Index 8 (100% over 50bp)
CGGGGAGAGTCGGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1503650.214671243692957Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCACCGCTGGTCTAATGGTAGAATTTCAGATCGGAAGAGCACACGTCTGAA1501770.21440284217788186Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGGGGAGAGTCGGATACTTTGAGAGTAGATCGGAAGAGCACACGTCTGAA1497390.21377752375446207Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGAGATCGGAAGAGCACACGTC1420720.2028315960093492No Hit
GCCTGTGTAGCTCAACCGGATAGAGCATAAGAAGATCGGAAGAGCACACGT1397240.19947943240617647No Hit
GCACCGCTGGTCTAATGGTAGAATTTCACAAGATCGGAAGAGCACACGTCT1357780.19384585592486495Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGATATCGTTCAGCGGTTAGGATATAAGATCGGAAGAGCACACGTCTGA1348710.19255096138139063Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGAGAGTCGGATACTTTGAGAGTAGTGTCTTAAGGGGGTGGTATGTCC1286050.183605195990641No Hit
AGTTCGATTCTGGCCTGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT1261560.18010883795649707Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATGTTGGACCGATACGAATAATCATACTCAAAAGGTAGATCGGAAGAGCA1257010.17945924918330988No Hit
CGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT1209230.17263785323102743TruSeq Adapter, Index 8 (100% over 43bp)
GCACCGCTGGTCTAATGGTAGAATTTCAAGATCGGAAGAGCACACGTCTGA1125240.16064687277828146Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGTTGGACCGATACGAATAATCATACTCAAAAGGTAGATCGGAAGAGCAC1115180.15921063913910272No Hit
GGGACTTTAGCTCACCTGGGAGAGCGTGAGATCGGAAGAGCACACGTCTGA1083070.15462639836832437Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGACTTTAGCTCACCTGGGAGAGCGTGCGAGATCGGAAGAGCACACGTCT1048310.14966382567470074Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTTTATGACTGACAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1013670.14471838499267764Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC1008160.1439317401266861TruSeq Adapter, Index 8 (100% over 44bp)
TTCGGGTCTTTCAGGATATTAGATCGGAAGAGCACACGTCTGAACTCCAGT1000530.14284242972241828Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGGTGCTTGCTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC995920.14218427494343083TruSeq Adapter, Index 5 (100% over 35bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGACTCTTAATCTAGATCGGAA978970.13976437830485428No Hit
TTCGGGTCTTTCAGGATATTGTTAGATCGGAAGAGCACACGTCTGAACTCC966150.13793410839886303Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGAGAGTCGGATACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA946230.13509019447317308Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT921040.13149389970469266TruSeq Adapter, Index 8 (100% over 43bp)
GGAAACTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAT852740.12174292976871756TruSeq Adapter, Index 8 (100% over 41bp)
GGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC846030.12078496478672059TruSeq Adapter, Index 8 (100% over 44bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGAAGATCGGAAGAGCACACGTC792950.11320690498874755No Hit
CGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC786880.11234031073528682TruSeq Adapter, Index 8 (100% over 44bp)
TACTTGGTTGATCCTGCCAAGGAAGCACACTCAGATCGGAAGAGCACACGT744690.10631698099006295No Hit
CCGACCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATC732590.10458950315367498TruSeq Adapter, Index 8 (100% over 42bp)
GCTTGACTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA724610.10345022438223894TruSeq Adapter, Index 8 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)