FastQCFastQC Report
Mon 11 Sep 2023
HWNHNBGXT_n01_3688_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNHNBGXT_n01_3688_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56626261
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGATATCGTTCAGCGGTTAGGATATGTGAGATCGGAAGAGCACACGTCT26816824.73575678959273Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCAGATCGGAAGAGCACACGTCT18297203.2312216411392582Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGATATCGTTCAGCGGTTAGGATATGTAGATCGGAAGAGCACACGTCTG14018282.475579307629017Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGATATCGTTCAGCGGTTAGGATATGAGATCGGAAGAGCACACGTCTGA11403412.013802394616872Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGGAGATCGGAAGAGCACACGTC10148541.7921967335968025No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGGC8107901.4318268338430469No Hit
AAGTCTCTAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG5315600.9387164022713772TruSeq Adapter, Index 3 (100% over 38bp)
GGGACTTTAGCTCACCTGGGAGAGCGTGCGAAGATCGGAAGAGCACACGTC3259890.5756851931297389No Hit
TACATCTTTTTTAATCGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGT2865980.5061220623413578Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTTTTATGACTGACAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2295270.40533666879400704Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCACCGCTGGTCTAATGGTAGAATTTCACTAGATCGGAAGAGCACACGTCT1842470.32537376960135156Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGATATCGTTCAGCGGTTAGGATATGCAGATCGGAAGAGCACACGTCTG1742150.30765760783675966Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGATATCGTTCAGCGGTTAGGATATGTAAGATCGGAAGAGCACACGTCT1535430.2711515775339643Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGATCGGAAGAGCACACGTCTG1390580.24557157323172019Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGGGAGAGTCGGATACTTTGAGAGTAGATCGGAAGAGCACACGTCTGAA1275480.22524531506680265Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCACCGCTGGTCTAATGGTAGAATTTCAGATCGGAAGAGCACACGTCTGAA1212680.21415505431305096Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTTGGACCGATACGAATAATCATACTCAAAAGGTAGATCGGAAGAGCACA1052380.18584663394957332No Hit
GCCTGTGTAGCTCAACCGGATAGAGCATAAGAAGATCGGAAGAGCACACGT1002110.17696912745130744No Hit
GGGACTTTAGCTCACCTGGGAGAGCGTGCGAGATCGGAAGAGCACACGTCT957390.1690717315769798Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC945790.16702321207469445TruSeq Adapter, Index 3 (100% over 50bp)
TTACCTAATCGGAAATGCTTAAATGAATTAGCGTTACAAGTGGGCTCTGAC872860.15414402868661944No Hit
GATGTTGGACCGATACGAATAATCATACTCAAAAGGTAGATCGGAAGAGCA870970.153810261284954No Hit
GCACCGCTGGTCTAATGGTAGAATTTCACAAGATCGGAAGAGCACACGTCT855260.15103593013142788Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACTTGGTTGATCCTGCCAAGGAAGCACACTTAGATCGGAAGAGCACACGT841590.1486218558558899No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTGAAGATCGGAAGAGCACACGTC801370.14151914427124193No Hit
GCCTTTCGGTACTGTGGATAGGGGTAGATCGGAAGAGCACACGTCTGAACT758530.13395374983349156Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCACCGCTGGTCTAATGGTAGAATTTAGATCGGAAGAGCACACGTCTGAAC738230.13036884070449223Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCAAGATCGGAAGAGCAC722210.12753976463323263No Hit
CCACAGGATCGGGGTTCGACTCCCCGTATCGGAACCAAGATCGGAAGAGCA709310.12526166966948427No Hit
GCACCGCTGGTCTAATGGTAGAATTTCAAGATCGGAAGAGCACACGTCTGA704780.12446168748454008Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGATACCGTTCAGCGGTTAGGATATGTGGAGATCGGAAGAGCACACGTC693910.12254208343369166No Hit
GCCTGTGTAGCTCAACCGGATAGAGCATAAGAGATCGGAAGAGCACACGTC687200.12135712085952488No Hit
GCCTGTGTAGCTCAACCGGATAGAGCATAAGACTCTTAATCTAGATCGGAA651100.11498198689120583No Hit
CGGGGAGAGTCGGATACTTTGAGAGTAGATCGGAAGAGCACACGTCTGAAC643310.11360630008751595Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACCGCTGGTCTAATGGTAGAATTAGATCGGAAGAGCACACGTCTGAACT621510.10975649619529003Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGACTTTAGCTCACCTGGGAGAGCGTGCAGATCGGAAGAGCACACGTCTG617140.10898476945175667Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGACTTTAGCTCACCTGGGAGAGCGTGAGATCGGAAGAGCACACGTCTGA600150.1059843947669439Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACCGCTGGTCTAATGGTAGAATTTCACAGATCGGAAGAGCACACGTCTG573880.10134520448030286Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)