FastQCFastQC Report
Mon 11 Sep 2023
HWNHNBGXT_n01_3688_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNHNBGXT_n01_3688_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58624826
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGATATCGTTCAGCGGTTAGGATATGTGAGATCGGAAGAGCACACGTCT25339734.322354833087266Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCAGATCGGAAGAGCACACGTCT21692123.7001593829890425Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGATATCGTTCAGCGGTTAGGATATGTAGATCGGAAGAGCACACGTCTG12893122.1992594059042494Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC11845682.020591071775633TruSeq Adapter, Index 2 (100% over 50bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGGAGATCGGAAGAGCACACGTC10377831.7702107977258643No Hit
TCCGATATCGTTCAGCGGTTAGGATATGAGATCGGAAGAGCACACGTCTGA9417661.6064286485046455Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGTCTCTAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG7315451.2478416567070068TruSeq Adapter, Index 2 (100% over 38bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGGC7068121.2056530453497636No Hit
TTTTTATGACTGACAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6980901.1907753892523278Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGACTTTAGCTCACCTGGGAGAGCGTGCGAAGATCGGAAGAGCACACGTC3663950.6249826651937526No Hit
TACATCTTTTTTAATCGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGT3069560.5235938781293781Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGTTGGACCGATACGAATAATCATACTCAAAAGGTAGATCGGAAGAGCACA1934520.32998306894761614No Hit
GCACCGCTGGTCTAATGGTAGAATTTCACTAGATCGGAAGAGCACACGTCT1907400.3253570424243135Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGGGGAGAGTCGGATACTTTGAGAGTAGATCGGAAGAGCACACGTCTGAA1796760.3064844917407516Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGATATCGTTCAGCGGTTAGGATATGCAGATCGGAAGAGCACACGTCTG1745410.2977254039099408Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGAATGCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG1569020.26763746812655786TruSeq Adapter, Index 2 (100% over 38bp)
GATGTTGGACCGATACGAATAATCATACTCAAAAGGTAGATCGGAAGAGCA1469460.2506549017305399No Hit
GCCTGTGTAGCTCAACCGGATAGAGCATAAGATCGGAAGAGCACACGTCTG1443480.24622333207436728Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGATATCGTTCAGCGGTTAGGATATGTAAGATCGGAAGAGCACACGTCT1353050.2307981263773815Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCAGATCGGAAGAGCACACGTCTGAA1266670.21606375428730484Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGAAGATCGGAAGAGCACACGT1237810.21114092517733019No Hit
GCCTTTCGGTACTGTGGATAGGGGTAGATCGGAAGAGCACACGTCTGAACT1131590.19302232129439498Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGACTTTAGCTCACCTGGGAGAGCGTGCGAGATCGGAAGAGCACACGTCT1033840.17634849781899567Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGACTCTTAATCTAGATCGGAA1002910.17107257597660078No Hit
GCACCGCTGGTCTAATGGTAGAATTTCACAAGATCGGAAGAGCACACGTCT912690.15568319128145472Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTACCTAATCGGAAATGCTTAAATGAATTAGCGTTACAAGTGGGCTCTGAC874380.1491484170886921No Hit
ATGTTGGACCGATACGAATAATCATACTCAAAAGGTAGATCGGAAGAGCAC840070.14329594769287674No Hit
GCCTGTGTAGCTCAACCGGATAGAGCATAAGAGATCGGAAGAGCACACGTC839660.14322601145118963No Hit
TTTTTATGACTGACAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC825190.14075777384823285Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGAAGATCGGAAGAGCACACGTC801410.13670147183038123No Hit
TGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCAAGATCGGAAGAGCAC800850.1366059491588086No Hit
GCACCGCTGGTCTAATGGTAGAATTTAGATCGGAAGAGCACACGTCTGAAC793520.13535562561840267Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCACAGGATCGGGGTTCGACTCCCCGTATCGGAACCAAGATCGGAAGAGCA769910.13132832155442134No Hit
GCACCGCTGGTCTAATGGTAGAATTTCAAGATCGGAAGAGCACACGTCTGA762460.130057528870107Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACTTGGTTGATCCTGCCAAGGAAGCACACTTAGATCGGAAGAGCACACGT729490.124433631581269No Hit
TTGGACCGATACGAATAATCATACTCAAAAGGTAGATCGGAAGAGCACACG729200.12438416448349032No Hit
TTTTTATGACTGACAGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA721890.12313725246706915Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCGGGTCTTTCAGGATATTGTTAGATCGGAAGAGCACACGTCTGAACTCC721480.12306731622538207Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGAGAGTCGGATACTTTGAGAGTAGATCGGAAGAGCACACGTCTGAAC720440.12288991697817575Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACCGCTGGTCTAATGGTAGAATTTCACAGATCGGAAGAGCACACGTCTG702160.11977178405612667Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACCGCTGGTCTAATGGTAGAATTAGATCGGAAGAGCACACGTCTGAACT694510.11846687613196498Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGACTTTAGCTCACCTGGGAGAGCGTGCAGATCGGAAGAGCACACGTCTG595460.10157130359755778Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)