FastQCFastQC Report
Mon 11 Sep 2023
HWNHNBGXT_n01_3688_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNHNBGXT_n01_3688_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64001935
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGATATCGTTCAGCGGTTAGGATATGTGAGATCGGAAGAGCACACGTCT13502712.1097346509914114Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCAGATCGGAAGAGCACACGTCT11884001.8568188602422722Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGATATCGTTCAGCGGTTAGGATATGTAGATCGGAAGAGCACACGTCTG10262801.6035140187558392Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGATATCGTTCAGCGGTTAGGATATGAGATCGGAAGAGCACACGTCTGA6214080.9709206448211293Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGGC5223290.8161143877915565No Hit
GATGTTGGACCGATACGAATAATCATACTCAAAAGGTAGATCGGAAGAGCA4772070.7456133943450304No Hit
TGTTGGACCGATACGAATAATCATACTCAAAAGGTAGATCGGAAGAGCACA4727490.7386479799399815No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTGGAGATCGGAAGAGCACACGTC4512130.7049989972959412No Hit
ATGTTGGACCGATACGAATAATCATACTCAAAAGGTAGATCGGAAGAGCAC2731080.426718348437434No Hit
GCCTTTCGGTACTGTGGATAGGGGTAGATCGGAAGAGCACACGTCTGAACT2189620.34211778128270653Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTACCTAATCGGAAATGCTTAAATGAATTAGCGTTACAAGTGGGCTCTGAC2085400.32583389861572154No Hit
TTTTTATGACTGACAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1944620.30383768865738203Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGTCTCTAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC1929150.30142057423732577TruSeq Adapter, Index 1 (100% over 38bp)
TACATCTTTTTTAATCGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGT1918690.2997862486501385Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCACCGCTGGTCTAATGGTAGAATTTCAGATCGGAAGAGCACACGTCTGAA1828980.2857694849382288Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCACCGCTGGTCTAATGGTAGAATTTCAAGATCGGAAGAGCACACGTCTGA1490300.23285233485518833Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGACTTTAGCTCACCTGGGAGAGCGTGCGAAGATCGGAAGAGCACACGTC1407380.21989647656746628No Hit
ATCACAAGGAATAGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1381990.2159294090092745Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGGCGTAGAGAAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1255050.19609563367107571Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGGGGAGAGTCGGATACTTTGAGAGTAGATCGGAAGAGCACACGTCTGAA1187580.1855537648978894Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAAGACATTGTGTGAACGAAGGTACTGTAAGCAGTGGAGTAGATCGGAAG1165740.18214136806957476No Hit
ATCTCGGTCGAGAAGCATGGGTGTGACAGTACTACAAGATCGGAAGAGCAC1151790.1799617464690716No Hit
TCCGATATCGTTCAGCGGTTAGGATATGCAGATCGGAAGAGCACACGTCTG1099670.1718182426828189Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACCGGATGCTTACGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1066160.1665824634833306Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGGAATGCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC1034500.16163573804448256TruSeq Adapter, Index 1 (100% over 38bp)
ATGTAGAAGACATTGTGTGAACGAAGGTACTGTAAGCAGTGGAGTAGATCG963160.15048920005309216No Hit
GCCTGTGTAGCTCAACCGGATAGAGCATAAGATCGGAAGAGCACACGTCTG898800.14043325408833343Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTCTAAGTCAGATGGAATGCTAGATCGGAAGAGCACACGTCTGAACTCCA841080.13141477675635901Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCGGGTCTTTCAGGATATTGTTAGATCGGAAGAGCACACGTCTGAACTCC821140.12829924595248565Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTTTTACGTTCACTGTGAACAAATCAGGACGCTTAGAGTATGGCTACATG819700.12807425275501436No Hit
GTCGATCAAGGGCGAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC760140.11876828411515995Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTTAAATGGATAGCAGCAGCAACTCTGGTAGATCGGAAGAGCACACGTCTG715580.11180599461563154Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTAGAAGACATTGTGTGAACGAAGGTACTGTAAGCAGTGGAGTAGATCGGA704140.11001854865794292No Hit
GCCTTTCGGTACTGTGGATAGGGGTAAGATCGGAAGAGCACACGTCTGAAC691440.10803423365246692Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCTTTCGGTACTGTGGATAGGGGTACGGTTTTCCACCGTAGATCGGAAGA675000.10546556131466962No Hit
CGAGAAGCATGGGTGTGACAGTACTACAAGATCGGAAGAGCACACGTCTGA650940.10170629997358674Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGACCGATACGAATAATCATACTCAAAAGGTAGATCGGAAGAGCACACG645520.10085945057754894No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)