FastQCFastQC Report
Wed 29 Nov 2017
HWNGLBGX3_n02_hbepc_b2_mock_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNGLBGX3_n02_hbepc_b2_mock_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37012158
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG202350.032.10601450
TATAGGG86700.028.25971648
ATAGGGG111750.025.2561349
GGGGAGA581650.022.287654
GGGGGGT811700.021.45862414
GGGAAAA456800.021.2285322
GGGAGAG586300.021.131265
GGGGGGA818900.018.9356062
AGAGGGG440600.017.8114998
GGGGGAG772450.017.722713
GTTTGGT240450.017.62261831
GTTGGGA210750.017.44013819
GAGAGGG447200.017.307427
TGGGGAA433950.016.78867720
AGAGCGG158450.016.6980868
GAATTAA153750.016.66222466
GGGTGGG849750.015.68520517
GGTTTGG293400.015.6318135
GTAGGGG189500.015.458220549
GGGGGTT353200.015.15404215
Produced by FastQC (version 0.11.2)