FastQCFastQC Report
Wed 29 Nov 2017
HWNGLBGX3_n02_hbepc_b2_mock_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNGLBGX3_n02_hbepc_b2_mock_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39579741
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGGG125800.038.5420948
TAGGGGG254850.038.52982750
ATAGGGG147900.035.57749649
GGGAAAA619000.027.52452322
GGGGAGA664100.027.3447284
GGGAGAG659550.025.481695
GGGGGGT835700.025.06172214
GTTGGGA256500.023.70779219
TTATAGG109900.022.60978147
GGGTTGG404500.020.97154417
GGGGGGA900700.020.9357452
TTGGGAA348350.020.66664320
CGGGTAA49400.020.66162761
ATATAGG72050.020.64452447
GGGGGTT375700.020.427715
GGTTGGG398550.020.31478718
GGGGTTG415950.020.29284116
GTTTGGT265150.020.23612231
AGAGCGG187350.020.2358558
GGGGGAG803950.019.6824473
Produced by FastQC (version 0.11.2)